
The screening of patient urine samples for basic drugs is typically performed following a lengthy sample preparation methodology that requires a time consuming dry down step.
A novel elution protocol coupled to the EVOLUTE EXPRESS advanced plate technology affords a fast sample preparation solution that follows a LOAD-WASH-ELUTE-ANALYZE strategy. Basic drugs are fortified into urine and extracted in 3 easy steps using EVOLUTE EXPRESS CX solid phase extraction in a 96-well plate format.
Tags:
6-acetyl codeine,
6-acetylmorphine (MAM),
96-well plate,
Alprazolam,
Application Notes,
Basic,
Buprenorphine,
Clinical,
Clonazepam,
Codeine,
Diazepam,
Drugs of Abuse,
EDDP,
English,
Fentanyl,
Flunitrazepam,
Forensic,
Hydrocodone,
Hydromorphone,
LC-MS/MS,
Morphine,
Naltrexone,
Nitrazepam,
Normeperidine,
Oxazepam,
Oxycodone,
Oxymorphone,
Pain Management,
Temazepam,
Urine
This application note describes a method of extraction for metanephrine and normetanephrine from synthetic
urine using EVOLUTE® EXPRESS WCX 96-well SPE plates.
A new solid phase extraction technology has been developed to reduce
the time associated with sample preparation methods by
eliminating steps in the extraction workflow. Leveraging this
technology, a method protocol was developed using a load,
wash and elute approach. This method was optimized for
synthetic urine.
Tags:
96-well plate,
Application Notes,
Catecholamines,
Clinical,
English,
LC-MS/MS,
Metanephrine,
Normetanephrine,
Synthetic urine
This application note describes a method of extraction for metanephrine and normetanephrine from pooled
plasma using EVOLUTE® EXPRESS WCX 96-well SPE plates.
A new solid phase extraction
technology has been developed to reduce the time
associated with sample preparation methods by eliminating
traditional steps in the extraction workflow. Leveraging this
technology, a method protocol was developed using a load,
wash and elute approach. This method was optimized for
pooled plasma.
Tags:
96-well plate,
Application Notes,
Catecholamines,
Clinical,
English,
LC-MS/MS,
Metanephrine,
Normetanephrine,
Plasma
In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing.
Tags:
Catalogs
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags:
96-well plate,
Clinical,
Column,
English,
Environmental,
Food,
Forensic,
Manuals & User Guides,
On-line SPE,
Pharma
QuickStart Guide to SPE
Tags:
English,
Manuals & User Guides
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
ASMS 2017
Tags:
11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH),
6-acetylmorphine (MAM),
7-Aminoclonazepam,
96-well plate,
Alprazolam,
Amitriptyline,
Amphetamine,
Amphetamines,
Analgesics,
Barbiturates,
Benzodiazepines,
Benzoylecgonine,
Buprenorphine,
Butalbital,
Cannabinoid,
Carbamate hypnotic,
Carbamate muscle relaxant,
Carisoprodol,
Chlordiazepoxide,
Clinical,
Clonazepam,
Cocaine,
Cocaines,
Codeine,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
EDDP,
English,
Fentanyl,
Forensic,
Gabapentin,
Hydrocodone,
Hydrolysed urine,
Hydromorphone,
Ketamine,
LC-MS/MS,
Lorazepam,
MDMA,
Meperidine,
Meprobamate,
Methadone,
Methamphetamine,
Morphine,
N-des-tapentadol,
Naloxone,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norhydrocodone,
Norketamine,
Normeperidine,
Nortryptiline,
Opiates,
Opioid,
Oxazepam,
Oxycodone,
Oxymorphone,
Pain Management,
Phencyclidine,
Phenobarbital,
Posters,
Pregabalin,
Ritalinic acid,
Secobarbital,
Tapentadol,
Temazepam,
Tramadol,
Urine,
Zolpidem,
Zolpidem-phenyl-4-COOH,
z-drugs,
α-OH alprazolam
This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection.
ASMS 2017
Tags:
96-well plate,
Clinical,
English,
Oxytocin,
Peptides,
Pharma,
Posters,
Serum,
Vasopressin
This poster discusses the impact of optimization of various parts of the method development process to maximize assay performance while delivering a combined assay for the analysis of urinary catecholamines and metanephrines. The extraction method using EVOLUTE EXPRESS WCX 96-well plates is successfully automated using the Biotage® Extrahera™ Automated Sample Preparation Platform.
MSACL 2017, Palm Springs
ASMS 2017, Indianapolis
Tags:
3-methoxytyramine,
96-well plate,
Catecholamines,
Clinical,
Dopamine,
English,
Epinephrine,
Extrahera,
LC-MS/MS,
Metanephrine,
Metanephrines,
Norepinephrine,
Normetanephrine,
Posters,
Urine
Part of the B complex group of water soluble vitamins, vitamin B7 (Biotin) is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize
sensitivity allowing low level analysis of vitamin B7 from human serum.
MSACL 2017, Palm Springs
ASMS 2017, Indianapolis
Tags:
96-well plate,
Biotin,
Clinical,
English,
LC-MS/MS,
Posters,
Serum,
Vitamin B7,
Vitamins,
Water Soluble Vitamins
This poster describes a simple solid phase extraction method using EVOLUTE® EXPRESS ABN columns for the extraction of the marine toxins okadaic acid (OA), dinophysistoxins (DTX1 and DTX2), ciguatoxin 3C (CTX3C) and tetrodotoxin (TTX).
ASMS 2017
Tags:
Ciguatoxin 3C (CTX3C),
Column,
Diarrhetic Shellfish Poisoning (DSP) Toxins,
Dinophysistoxin 1 (DTX1),
Dinophysistoxin 2 (DTX2),
English,
Food,
LC-MS/MS,
Marine Toxins,
Okadaic Acid (OA),
Paralytic Shellfish Poisoning (PSP) Toxins,
Posters,
Tetrodotoxin (TTX)
This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples.
The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060).
ASMS 2017
Tags:
96-well plate,
Amikacin,
Aminoglycoside antibiotics,
Apramycin,
Dihydrostreptomycin,
Eggs,
English,
Extrahera,
Food,
Gentamicin,
Gentamicin C1,
Gentamicin C1a,
Gentamicin C2,
Hygromycin B,
Kanamycin,
LC-MS/MS,
Meat,
Milk,
Neomycin,
Netilmicin,
Paromomycin,
Posters,
Sisomycin,
Spectinomycin,
Streptomycin,
Tobramycin,
Veterinary drugs
This poster compares the use of anion and cation exchange SPE approaches for the extraction of these important clinical biomarkers.
MSACL Europe 2014
Tags:
96-well plate,
Clinical,
English,
LC-MS/MS,
Plasma,
Posters,
Reverse Tri-iodothyronine (rT3),
Thyroid Hormone,
Thyroxine (T4),
Tri-iodothyronine (T3),
rT3
This report details a “load, wash, elute” weak cation exchange solid phase extraction procedure amenable to both plasma and urine samples. The extracts are subsequently injected into an LC-MS/MS system. The preliminary sample preparation method was developed at the Biotage US Applications lab (Charlotte, NC).The method was then transferred to Ionics (Bolton, ON, Canada) to facilitate the nmole/L measurements of the selected biomarkers
by laminar flow tandem mass spectrometry. The SPE-LC-ESIMS/ MS method parameters were first optimized using pooled mixed gender plasma. A set of patient samples (n=32) was later supplied by the Mayo Clinic (Rochester, MN) that had previously been analyzed by a validated referee method. The population represented measured values across a range of clinical relevance.
AACC 2014
Tags:
96-well plate,
Catecholamines,
Clinical,
English,
LC-MS/MS,
Metanephrine,
Normetanephrine,
Plasma,
Posters,
Urine
This poster evaluates the performance of various polymer-based
ion exchange SPE chemistries for the extraction of thyroid
hormones prior to LC-MS/MS analysis.
Tags:
96-well plate,
Clinical,
English,
LC-MS/MS,
Posters,
Reverse Tri-iodothyronine (rT3),
Serum,
T3,
T4,
Thyroid Hormone,
Thyroxine (T4),
Tri-iodothyronine (T3),
rT3
This poster describes a reduced workflow solid phase extraction method for extraction of vitamin B7 (biotin) from human serum. Using EVOLUTE EXPRESS AX 96-well plates, the traditional sorbent conditioning and equilibration steps are not required, meaning the sample can be applied directly to the dry plate. Post extraction evaporation is also eliminated.
ASMS 2014
Tags:
96-well plate,
Biotin,
Clinical,
English,
LC-MS/MS,
Posters,
Serum,
Vitamin B7,
Vitamins
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
HPLC 2014
Tags:
96-well plate,
Clinical,
English,
Forensic,
LC-MS/MS,
Pharma,
Posters
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
HPLC2014
Tags:
Biological fluids,
English,
HPLC,
LC-MS/MS,
Plasma,
Posters
This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices.
Pittcon 2014
Tags:
96-well plate,
ACTH (1-24),
Angiotensin Fragment 1-7,
Angiotensin II,,
Clinical,
Elafin,
Endorphin,
English,
Glycoprotein myelin,
Helicobacter pylori (2-20),
LC-MS/MS,
Peptides,
Pharma,
Plasma,
Posters,
Serum,
Vasoactive Intestinal Fragment 1-12
In this poster, an IONICS 3Q 220 triple quadrupole mass spectrometer using a load, wash, elute method protocol with recently advanced solid phase extraction (SPE) sorbent technology (EVOLUTE EXPRESS WCX) was used to perform analysis of the two compounds. As a result low levels of metanephrine and normetanephrine are detectable in plasma with short sample preparation and LC run times. This LC-MS/MS method provides a fast, sensitive, accurate, and reproducible solution for the analysis of ME and NME in plasma.
MSACL 2014
Tags:
96-well plate,
Clinical,
English,
LC-MS/MS,
Metanephrine,
Normetanephrine,
Plasma,
Posters
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags:
ACTH (1-24),
Alpha-Endorphin,
Angiotensin Fragment 1-7,
Angiotensin II,,
Elafin,
Helicobacter pylori (2-20),
LC-MS/MS,
Myelin Glycoprotein fragment (35-55) rat,
Peptides,
Pharma,
Plasma,
Posters,
Vasoactive Intestinal Fragment 1-12
This poster provides mixed-mode resin-based SPE sample preparation strategies for the simultaneous extraction of EtG and EtS and was presented at EBF 2012.
EtG, EtS, Poster, EBF, EBF 2012, Barcelona, LC-MS/MS, Ethyl Glucuronide, EVOLUTE, EVOLUTE EXPRESS, Polymeric, Polymer, SPE, Solid Phase Extraction,
Tags:
96-well plate,
Alcohol metabolite,
Column,
Ethyl Sulfate (EtS),
Ethyl glucuronide (EtG),
Forensic,
LC-MS/MS,
Posters,
Urine
This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches.
EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
Tags:
96-well plate,
Alcohol metabolite,
Column,
Ethyl Sulfate (EtS),
Ethyl glucuronide (EtG),
Forensic,
LC-MS/MS,
Posters,
Urine
EXPRESS represents a leap forward in high
throughput solid phase extraction. The EVOLUTE
EXPRESS range of 96-well SPE plates combines
powerful EVOLUTE sorbent chemistry with innovative
features that enhance productivity by optimizing
or even eliminating the need for some traditional
steps in the SPE procedure. By truly eliminating the
need for conditioning and equilibration, samples
can be prepared by loading the plate, washing away
interferences and eluting with compatible solvents
Tags:
English,
Product Notes
Plasma renin activity (PRA) determination is used in the diagnosis of primary aldosteronism, an important cause of secondary hypertension (high blood pressure). Concomitant measurement of both aldo...
13 August 2018Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...
24 October 2017This application note from Biotage describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis. Desmopressi...
05 July 2017This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolit...
29 May 2017Polyfluorinated compounds (PFCs) such as PFOS (perfluorooctanesulfonic acid) and PFOA (perfluorooctanoic acid) are man-made fluorosurfactants. Due to their widespread industrial use and chemical st...
19 May 2017The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Was...
20 April 2017Vitamin B7 (Biotin) is a water soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum whi...
15 October 2015A new application note from Biotage describes an optimized method for extraction of the catecholamines dopamine, epinephrine and norepinephrine from human plasma. The extraction method utilizes the...
06 October 2015Order Two Boxes and Receive a Third Box at no Charge Speed up your sample preparation with EVOLUTE® EXPRESS. Simply... Load Wash Elute The streamlined three-step protocol eliminates c...
26 June 2015SPE without conditioning or equilibration is a reality with the water wettable sorbent chemistries in EVOLUTE® EXPRESS. The result: faster work flow, higher throughput and increased lab ef...
16 June 2015Uppsala, Sweden, 16th June 2015 – Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, announces the addition of new polymer...
15 April 2015The time saving benefits of EVOLUTE® EXPRESS SPE plates and columns are explained in this video from Biotage analytical labs. Product manager Claire Desbrow explains the protein ...
27 March 2015Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
23 October 2014A simplified method for screening metanephrine and normetanephrine proves as effective as the current gold standard. It is anticipated that this time-saving and sensitive SPE-LC-ESI-MS/MS...
29 July 2014Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
10 July 2014Eight new posters by Biotage Scientists and collaborating groups have been presented at ASMS 2014, Baltimore, MD, USA. P81 A Reduced Workflow Method For The Extraction Of Vitamin B7 (bi...
30 June 2014Biotage is attending EPRW (European Pesticides and Residues Workshop) 2014 30th June - 3rd July 2014, Dublin, Ireland Read more at conference website http://eprw2014.com/
20 May 2014Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sa...
30 April 2014The method described in this application note demonstrates selective extraction of the thyroid hormones T3, rT3 and T4 from serum. 500 μL of serum was extracted using the EVOLUTE® EXPRESS AX...
01 April 2014Biotage is attending the Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT) 1st - 3rd April 2014, Hannover, Germany Read more at the conference ...
11 January 2014Biotage is attending the 22nd Congress French Toxicology Society19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society 11th-13th June 2014, Bordeuax, Fran...
28 August 2012Uppsala, Sweden, 28th August, 2012 Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, introduced EVOLUTE® EXPRESS, a novel ...
28 August 2012EVOLUTE EXPRESS 96-Well SPE EVOLUTE EXPRESS® represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combine powerful EVOLUTE sorbe...
18 April 2011Uppsala, Sweden – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of sample preparat...
16 December 2010Biotage Announces an Easy, Cost-Effective Approach to the Analysis of Ethyl Glucuronide (EtG) in Urine for Clinical and Forensic Toxicology Laboratories Uppsala, Sweden – Biotage®...
23 February 2010Uppsala, Sweden, March 2, 2010– Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of p...
Question:
Is there an expiry (‘best before’) date for ISOLUTE and EVOLUTE sample preparation products?
Answer:
There is no fixed expiry date for these products.
All ISOLUTE and EVOLUTE products are packaged in inner metal laminate barrier bags, which provide an effective barrier to oxygen and moisture penetration. Recommended storage conditions are standard (see below).
We have no indications that the performance of non-bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
We have no indications that the performance of bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
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