The screening of patient urine samples for basic drugs is typically performed following a lengthy sample preparation methodology that requires a time consuming dry down step. A novel elution protocol coupled to the EVOLUTE EXPRESS advanced plate technology affords a fast sample preparation solution that follows a LOAD-WASH-ELUTE-ANALYZE strategy. Basic drugs are fortified into urine and extracted in 3 easy steps using EVOLUTE EXPRESS CX solid phase extraction in a 96-well plate format.
Tags: 6-acetyl codeine, 6-acetylmorphine (MAM), 96-well plate, Alprazolam, Application Notes, Basic, Buprenorphine, Clinical, Clonazepam, Codeine, Diazepam, Drugs of Abuse, EDDP, English, Fentanyl, Flunitrazepam, Forensic, Hydrocodone, Hydromorphone, LC-MS/MS, Morphine, Naltrexone, Nitrazepam, Normeperidine, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Temazepam, Urine

This application note describes a method of extraction for metanephrine and normetanephrine from synthetic urine using EVOLUTE® EXPRESS WCX 96-well SPE plates. A new solid phase extraction technology has been developed to reduce the time associated with sample preparation methods by eliminating steps in the extraction workflow. Leveraging this technology, a method protocol was developed using a load, wash and elute approach. This method was optimized for synthetic urine.
Tags: 96-well plate, Application Notes, Catecholamines, Clinical, English, LC-MS/MS, Metanephrine, Normetanephrine, Synthetic urine

This application note describes a method of extraction for metanephrine and normetanephrine from pooled plasma using EVOLUTE® EXPRESS WCX 96-well SPE plates. A new solid phase extraction technology has been developed to reduce the time associated with sample preparation methods by eliminating traditional steps in the extraction workflow. Leveraging this technology, a method protocol was developed using a load, wash and elute approach. This method was optimized for pooled plasma.
Tags: 96-well plate, Application Notes, Catecholamines, Clinical, English, LC-MS/MS, Metanephrine, Normetanephrine, Plasma

In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing.
Tags: Catalogs

EVOLUTE® EXPRESS Solid Phase Extraction products from Biotage are designed for all your sample preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic toxicology, or with the diversity of samples in food safety and environmental applications, the tools and methods used for sample preparation have a clear impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags: 96-well plate, Clinical, Column, English, Environmental, Food, Forensic, Manuals & User Guides, On-line SPE, Pharma

QuickStart Guide to SPE
Tags: English, Manuals & User Guides

Tags: Manuals & User Guides

This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels. Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc). ASMS 2017
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetylmorphine (MAM), 7-Aminoclonazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Analgesics, Barbiturates, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Cannabinoid, Carbamate hypnotic, Carbamate muscle relaxant, Carisoprodol, Chlordiazepoxide, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Fentanyl, Forensic, Gabapentin, Hydrocodone, Hydrolysed urine, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Morphine, N-des-tapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Nortryptiline, Opiates, Opioid, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Phencyclidine, Phenobarbital, Posters, Pregabalin, Ritalinic acid, Secobarbital, Tapentadol, Temazepam, Tramadol, Urine, Zolpidem, Zolpidem-phenyl-4-COOH, z-drugs, α-OH alprazolam

This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection. ASMS 2017
Tags: 96-well plate, Clinical, English, Oxytocin, Peptides, Pharma, Posters, Serum, Vasopressin

This poster discusses the impact of optimization of various parts of the method development process to maximize assay performance while delivering a combined assay for the analysis of urinary catecholamines and metanephrines. The extraction method using EVOLUTE EXPRESS WCX 96-well plates is successfully automated using the Biotage® Extrahera™ Automated Sample Preparation Platform. MSACL 2017, Palm Springs ASMS 2017, Indianapolis
Tags: 3-methoxytyramine, 96-well plate, Catecholamines, Clinical, Dopamine, English, Epinephrine, Extrahera, LC-MS/MS, Metanephrine, Metanephrines, Norepinephrine, Normetanephrine, Posters, Urine

Part of the B complex group of water soluble vitamins, vitamin B7 (Biotin) is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize sensitivity allowing low level analysis of vitamin B7 from human serum. MSACL 2017, Palm Springs ASMS 2017, Indianapolis
Tags: 96-well plate, Biotin, Clinical, English, LC-MS/MS, Posters, Serum, Vitamin B7, Vitamins, Water Soluble Vitamins

This poster describes a simple solid phase extraction method using EVOLUTE® EXPRESS ABN columns for the extraction of the marine toxins okadaic acid (OA), dinophysistoxins (DTX1 and DTX2), ciguatoxin 3C (CTX3C) and tetrodotoxin (TTX). ASMS 2017
Tags: Ciguatoxin 3C (CTX3C), Column, Diarrhetic Shellfish Poisoning (DSP) Toxins, Dinophysistoxin 1 (DTX1), Dinophysistoxin 2 (DTX2), English, Food, LC-MS/MS, Marine Toxins, Okadaic Acid (OA), Paralytic Shellfish Poisoning (PSP) Toxins, Posters, Tetrodotoxin (TTX)

This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples. The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060). ASMS 2017
Tags: 96-well plate, Amikacin, Aminoglycoside antibiotics, Apramycin, Dihydrostreptomycin, Eggs, English, Extrahera, Food, Gentamicin, Gentamicin C1, Gentamicin C1a, Gentamicin C2, Hygromycin B, Kanamycin, LC-MS/MS, Meat, Milk, Neomycin, Netilmicin, Paromomycin, Posters, Sisomycin, Spectinomycin, Streptomycin, Tobramycin, Veterinary drugs

This poster compares the use of anion and cation exchange SPE approaches for the extraction of these important clinical biomarkers. MSACL Europe 2014
Tags: 96-well plate, Clinical, English, LC-MS/MS, Plasma, Posters, Reverse Tri-iodothyronine (rT3), Thyroid Hormone, Thyroxine (T4), Tri-iodothyronine (T3), rT3

This report details a “load, wash, elute” weak cation exchange solid phase extraction procedure amenable to both plasma and urine samples. The extracts are subsequently injected into an LC-MS/MS system. The preliminary sample preparation method was developed at the Biotage US Applications lab (Charlotte, NC).The method was then transferred to Ionics (Bolton, ON, Canada) to facilitate the nmole/L measurements of the selected biomarkers by laminar flow tandem mass spectrometry. The SPE-LC-ESIMS/ MS method parameters were first optimized using pooled mixed gender plasma. A set of patient samples (n=32) was later supplied by the Mayo Clinic (Rochester, MN) that had previously been analyzed by a validated referee method. The population represented measured values across a range of clinical relevance. AACC 2014
Tags: 96-well plate, Catecholamines, Clinical, English, LC-MS/MS, Metanephrine, Normetanephrine, Plasma, Posters, Urine

This poster evaluates the performance of various polymer-based ion exchange SPE chemistries for the extraction of thyroid hormones prior to LC-MS/MS analysis.
Tags: 96-well plate, Clinical, English, LC-MS/MS, Posters, Reverse Tri-iodothyronine (rT3), Serum, T3, T4, Thyroid Hormone, Thyroxine (T4), Tri-iodothyronine (T3), rT3

This poster describes a reduced workflow solid phase extraction method for extraction of vitamin B7 (biotin) from human serum. Using EVOLUTE EXPRESS AX 96-well plates, the traditional sorbent conditioning and equilibration steps are not required, meaning the sample can be applied directly to the dry plate. Post extraction evaporation is also eliminated. ASMS 2014
Tags: 96-well plate, Biotin, Clinical, English, LC-MS/MS, Posters, Serum, Vitamin B7, Vitamins

This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts. HPLC 2014
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Pharma, Posters

This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches. HPLC2014
Tags: Biological fluids, English, HPLC, LC-MS/MS, Plasma, Posters

This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices. Pittcon 2014
Tags: 96-well plate, ACTH (1-24), Angiotensin Fragment 1-7, Angiotensin II,, Clinical, Elafin, Endorphin, English, Glycoprotein myelin, Helicobacter pylori (2-20), LC-MS/MS, Peptides, Pharma, Plasma, Posters, Serum, Vasoactive Intestinal Fragment 1-12

In this poster, an IONICS 3Q 220 triple quadrupole mass spectrometer using a load, wash, elute method protocol with recently advanced solid phase extraction (SPE) sorbent technology (EVOLUTE EXPRESS WCX) was used to perform analysis of the two compounds. As a result low levels of metanephrine and normetanephrine are detectable in plasma with short sample preparation and LC run times. This LC-MS/MS method provides a fast, sensitive, accurate, and reproducible solution for the analysis of ME and NME in plasma. MSACL 2014
Tags: 96-well plate, Clinical, English, LC-MS/MS, Metanephrine, Normetanephrine, Plasma, Posters

This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012. EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags: ACTH (1-24), Alpha-Endorphin, Angiotensin Fragment 1-7, Angiotensin II,, Elafin, Helicobacter pylori (2-20), LC-MS/MS, Myelin Glycoprotein fragment (35-55) rat, Peptides, Pharma, Plasma, Posters, Vasoactive Intestinal Fragment 1-12

This poster provides mixed-mode resin-based SPE sample preparation strategies for the simultaneous extraction of EtG and EtS and was presented at EBF 2012. EtG, EtS, Poster, EBF, EBF 2012, Barcelona, LC-MS/MS, Ethyl Glucuronide, EVOLUTE, EVOLUTE EXPRESS, Polymeric, Polymer, SPE, Solid Phase Extraction,
Tags: 96-well plate, Alcohol metabolite, Column, Ethyl Sulfate (EtS), Ethyl glucuronide (EtG), Forensic, LC-MS/MS, Posters, Urine

This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches. EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
Tags: 96-well plate, Alcohol metabolite, Column, Ethyl Sulfate (EtS), Ethyl glucuronide (EtG), Forensic, LC-MS/MS, Posters, Urine

Tags: Posters

Tags: Column, English, Product Notes

EXPRESS represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combines powerful EVOLUTE sorbent chemistry with innovative features that enhance productivity by optimizing or even eliminating the need for some traditional steps in the SPE procedure. By truly eliminating the need for conditioning and equilibration, samples can be prepared by loading the plate, washing away interferences and eluting with compatible solvents
Tags: English, Product Notes