This application note describes the extraction of 18 growth promoters (steroids, resorcylic acid lactones and stilbenes) using ISOLUTE® HYDRO DME+ prior to LC/MS analysis. LOQs are typically < 0.4 ng/mL, with a load of 75 μL of urine.
Tags: 16-ß-hydroxystanozolol, Application Notes, Column, Dienestrol, Diethylstilbestrol, English, Food, Growth Promoters, Hexestrol, Hormone, Hormones, LC-MS/MS, Methylboldenone, Methyltestosterone, Steroid, Steroid Hormones, Steroids, Taleranol, Urine (Cattle), Urine (Pig), Urine (Sheep), Urine (horse), Veterinary drugs, Zearalenone, Zeranol, ß-nortestosterone, ß-zearalenol, α-boldenone, α-nortestosterone, α-trenbolone, α-zearalenol, β-boldenone, β-trenbolone
This application note describes the extraction of 25 illicit and prescribed drugs from hydrolyzed urine using ISOLUTE® HYDRO DME+ Dual Mode Extraction plates prior to UPLC-MS/MS analysis.
Because of the enhanced sample clean up delivered by ISOLUTE HYDRO DME+ products, analyte sensitivity is significantly increased compared to traditional dilute and shoot (D&S) techniques, resulting in reduced limits of quantitation (LOQ)
Tags: 6-MAM, 7-Aminoclonazepam, 7-amino-flunitrazepam, 96-well plate, Amphetamine, Amphetamines, Application Notes, Benzodiazepines, Benzoylecgonine, Buprenorphine, Clinical, Cocaine, Cocaines, Codeine, EDDP, Ecgonine Methyl Ester EME, Ecgonine methyl ester, English, Flunitrazepam, Forensic, Gabapentin, Hydrocodone, Hydrolysed urine, Ketamine, LC-MS/MS, MDMA, Mephedrone, Methadone, Morphine, Norbuprenorphine, Opioid, Oxazepam, Oxymorphone, PCP, Phencyclidine (PCP), Pregabalin, Ritalinic acid, Urine, Zaleplon
This poster evaluates two “pass through” solid phase extraction techniques: (protein removal/phospholipid depletion (ISOLUTE PLD+) and dual mode extraction (ISOLUTE HYDRO DME+)) and supported liquid extraction (ISOLUTE SLE+) for a group of about 100 drugs of abuse compounds in whole blood.
Tags: 96-well plate, Anticonvulsant, Antipsychotics, Barbiturates, Benzodiazepines, Cannabinoid, Clinical, English, Forensic, Hallucinogen, LC-MS/MS, Opiates, Opioid, Posters, Stimulants, Whole blood
This poster compares various sample preparation techniques (mixed-mode SPE (EVOLUTE EXPRESS CX, phospholipid depletion (ISOLUTE PLD+), supported liquid extraction (ISOLUTE SLE+) and dual mode extraction (ISOLUTE HYDRO DME+) for extraction of a panel of 98 drugs of abuse from urine.
Tags: 96-well plate, Amphetamines, Anticonvulsant, Antipsychotics, Barbiturates, Benzodiazepines, Cannabinoid, Clinical, English, Fentanyls, Forensic, Hallucinogen, LC-MS/MS, Opiates, Opioid, Posters, Stimulants, z-drugs
Sample preparation is generally regarded as a pain point for many labs as it can be laborious, time consuming and relatively expensive. In a high throughput setting performing offline chemistry also leads to increases in sample turnaround time and reporting.
This poster evaluates simplified sample preparation workflows using optimized sample hold-up technology allowing chemistry in-situ. Reduced labour, extraction complexity, processing time, solvent usage and resultant instrument contamination and downtime can offset upfront costs associated with sample preparation.
Tags: 96-well plate, Clinical, Drugs of Abuse, English, Forensic, Hydrolysed urine, LC-MS/MS, Over the counter pharmaceuticals, Pharmaceuticals, Plasma, Posters, Urine
This poster evaluates the extraction of a range of drugs of abuse from hydrolyzed and nonhydrolyzed urine using a novel flow through scavenging product, ISOLUTE® HYDRO DME+. Matrix component removal in terms of creatinine and urea, salt residue, pigmentation associated with urobillin content and protein removal will be demonstrated.
Tags: 96-well plate, Barbiturates, Benzodiazepines, Clinical, Cocaines, Column, Drugs of Abuse, English, Forensic, LC-MS/MS, Opiates, Opioid, Posters
Product Note for ISOLUTE® HYDRO DME+ Dual Mode Extraction 96-well Plates and Columns
Tags: 96-well plate, Amphetamines, Barbiturates, Benzodiazepines, Cannabinoid, Clinical, Cocaines, Column, Drugs of Abuse, English, Forensic, Hydrolysed urine, LC-MS/MS, Opiates, Opioid, Pain Management, Product Notes, Urine
These two application notes describe alternative approaches to the extraction of a suite of regulated veterinary growth promoters (including steroids, stilbenes and resorcylic acid lactones) from a...24 April 2019
This application note demonstrates the applicability of our newest sample preparation consumable, ISOLUTE® HYDRO DME+ for in-situ hydrolysis and clean-up of urine samples, for the analysis of a...13 February 2018
ISOLUTE® HYDRO DME+ is a new product targeted at drugs of abuse and pain management panels in urine applications. This novel patent pending product quickly and easily removes matrix components ...