
Reversed Phase chromatography using Isolera Dalton 2000
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Application Notes
The UV absorption spectrum of some solvents
overlaps with the product they dissolve, meaning
that fraction collection processes cannot distinguish
between solvent and product. Luckily, there is
technology that solves this problem.
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Application Notes
This application note outlines how to use the Isolera system to perform a size-exclusion separation as part of a sample clean up method defined by the EPA, a key sample preparation step in environmental laboratories.
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Application Notes
Summary of Biotage offerings for Mass Directed Flash Chromatography
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Brochures
Biotage has developed a range
of tools to improve the peptide synthesis workflow,
from synthesis of crude peptides through to the final
purified product. These solutions make your workflow
more efficient and improve the quality of peptides
you synthesize. Our workflow solutions are used in
pharma, biotech, CRO and academic laboratories
throughout the world.
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Brochures
Biotage has developed tools for every
step of the organic process, with the entire workflow in mind.
This dedicated suite of products vastly expands the
range of options in order to truly accelerate discoveries
of new molecules for future innovations.
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Brochures
This brochure presents the extensive range of instrumentation for flash purification from Biotage. To complement these products Biotage also offers a complete range of flash consumables, including columns in a variety of sizes packed with irregular and spherical silica, making Biotage your one-stop partner for your flash purification needs.
Keywords: Isolera™ Dalton, Isolera™ Spektra, 10 Isolera™ Spektra One and Four, Isolera™ Spektra LS (Large Scale), Isolera™ Prime, Isolera™ ELSD-1080, Biotage® Flash 75/150, Biotage® Flash 400
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Brochures
How to use the Atmospheric Solid Analysis Probe on Dalton 2000
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Manuals & User Guides
How to Install Isolera SW 2.3
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Manuals & User Guides
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
separation
» Isocratic separations are easiest to develop, but gradient separations are
more powerful
» Software in the Isolera helps with conversion of an isocratic separation to
a gradient
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
purifications
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Manuals & User Guides
Preparation, How Much are You Purifying?
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Manuals & User Guides
The Isolera leak detection system is designed with safety in
mind. Unlike vapor sensing devices with unreliable solvent
vapor sensitivity, the Isolera leak detector’s sensitive,
solvent-resistant refractive index (RI) monitor rapidly
signals the Isolera flash system to stop pumping if any
liquid, volatile or non-volatile, is detected thus protecting
the chemist, system, and lab from potential hazards.
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Manuals & User Guides,
Product Notes
Flash Chromatography is the primary tool used by medicinal chemists for reaction mixture purification. Over the past 10 or so years, the use of reversed-phase flash chromatography for reaction mixture purification has increased dramatically due to both chromatographic and non-chromatographic reasons.
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HPLC,
Pharma,
Posters
With the growing popularity and use of mass-directed flash
chromatography to purify specific compounds, it is important to understand that the sample’s compounds may not be detected as expected (e.g. M+H or M-H ions) because they may fragment or form adducts in the mass detector. This is true with both synthetic and natural product research.
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English,
Posters
New, higher default flow rates enabled by Isolera ACI systems decreases purification times compared to other systems’ and cartridges’ default flow rate settings without sacrificing separation efficiency. Incorporation of gradient optimization by converting a linear gradient to a step gradient further reduces purification time and solvent use while maintaining separation integrity for a target molecule.
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Posters
In this poster we demonstrate real-time detection and identification of peptides during reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system.
Presented at ACS Spring 2014.
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Posters
In order to obtain a fast synthesis of an N-unprotected tripeptide, microwave (mw) assisted deprotection, coupling and partial deprotection were applied starting with a purified protected dipeptide [Fig. 1]. The three consecutive mw assisted steps were performed without any intermediate purifications and were accomplished within some 30 minutes. The resulting crude mixture containing the Boc deprotected tripeptide ester was shown to be quite complex [Fig. 2]. The crude product was subjected to automated reversed phase flash chromatography, followed by automated fraction pooling and “thin film evaporation”. The total time for the entire procedure was 1 h 20 min. The purity of the product was > 99%.
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Posters
Bei herkömmlichen Aufreinigungsverfahren mittels Chromatographie kommen große Mengen verhältnismäßig giftiger und teurer Lösungsmittel zum Einsatz, die sich negativ auf den ökologischen Fußabdruck von Unternehmen in der Molekularforschung auswirken. Neue Richtlinien für einen verantwortungsvolleren Umgang mit Chemikalien zwingen Unternehmen, die Umweltauswirkungen ihrer Arbeit zu reduzieren. Durch Einblicke in die Arbeitsabläufe dieser Unternehmen und neue Entwicklungen im Bereich Flash-Chromatographiesysteme und -säulen lassen sich Verbrauch und Toxizität der für die Aufreinigung eingesetzten Lösungsmittel und somit auch die Umweltbelastungen dieser Verfahren reduzieren.
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Environmental,
German,
Product Notes,
White Papers
What is included in the Installation and Familiarization pachage for Isolera™ Dalton 2000?
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Product Notes
Modelle haben gezeigt, dass sich die zur Herstellung
eines Zielmoleküls benötigte Zeit mit dem automatisierten
Workflow von Biotage um bis zu achtzig
Prozent verkürzen lässt, sodass auch die Projektlaufzeiten
entsprechend verkürzt werden können.
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German,
Product Notes,
White Papers
Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced
accordingly.
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Product Notes,
White Papers
Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the
environmental impact of their work.
Tags:
Environmental,
Product Notes,
White Papers
With running costs and time a concern in most labs, ACI™ now promises to take users to the end point even faster and even more efficiently.
Tags:
Product Notes
The Isolera leak detection system is designed with safety in
mind. Unlike vapor sensing devices with unreliable solvent
vapor sensitivity, the Isolera leak detector’s sensitive,
solvent-resistant refractive index (RI) monitor rapidly
signals the Isolera flash system to stop pumping if any
liquid, volatile or non-volatile, is detected thus protecting
the chemist, system, and lab from potential hazards.
Tags:
Manuals & User Guides,
Product Notes
Use this simple tool to look up possible adducts and fragments which may occur with Isolera™ Dalton or Biotage® Dalton 2000 mass detectors.
Enter the target compound weight and use the mass lookup cell to highlight resulting m/z values.
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Software
Bei herkömmlichen Aufreinigungsverfahren mittels Chromatographie kommen große Mengen verhältnismäßig giftiger und teurer Lösungsmittel zum Einsatz, die sich negativ auf den ökologischen Fußabdruck von Unternehmen in der Molekularforschung auswirken. Neue Richtlinien für einen verantwortungsvolleren Umgang mit Chemikalien zwingen Unternehmen, die Umweltauswirkungen ihrer Arbeit zu reduzieren. Durch Einblicke in die Arbeitsabläufe dieser Unternehmen und neue Entwicklungen im Bereich Flash-Chromatographiesysteme und -säulen lassen sich Verbrauch und Toxizität der für die Aufreinigung eingesetzten Lösungsmittel und somit auch die Umweltbelastungen dieser Verfahren reduzieren.
Tags:
Environmental,
German,
Product Notes,
White Papers
Modelle haben gezeigt, dass sich die zur Herstellung
eines Zielmoleküls benötigte Zeit mit dem automatisierten
Workflow von Biotage um bis zu achtzig
Prozent verkürzen lässt, sodass auch die Projektlaufzeiten
entsprechend verkürzt werden können.
Tags:
German,
Product Notes,
White Papers
Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced
accordingly.
Tags:
Product Notes,
White Papers
Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the
environmental impact of their work.
Tags:
Environmental,
Product Notes,
White Papers
Want to improve your flash chromatography skills? Learn all the tricks of the trade in this one-hour webinar with flash guru Bob Bickler. For a dedicated synthetic chemist, the goal ...
25 September 2017In-line mass detection has become a powerful addition to the flash chemist's toolbox. While this technical advance in flash chromatography has helped chemists detect and isolate pure compounds...
13 July 2017Biotage is pleased to announce the release of the new Peptide Synthesis Workflow – Synthesis, Purification, and Evaporation Solutions Brochure The typical peptide synthesis workflow broadly ...
24 May 2017Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, is pleased to announce the launch of Isolera™ Dalton 2000, a new ma...
12 September 2014The capabilities of Biotage flash chromatography systems for providing pure compounds from natural products is confirmed in an extensive Korean study of active compounds in the medicinal herb&...
09 September 2014ACI™ - Accelerated Chromatographic Isolation radically improves the efficiency of the laboratory, embracing the latest developments in purification technology to guide chemists towards improv...
07 August 2014Flash Purification Has Come of Age Flash purification is a chromatographic technique developed with one goal in mind – to deliver the maximum amount of purified product in the minimum ...
27 June 2014Uppsala, Sweden, 27th June, 2014 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces the launch of ACI&trad...
14 March 2014By Dr. Greg Saunders, EU Marketing Manager for Organic and Peptide Chemistry at Biotage Liquid chromatography (LC) is used for two different purposes – analytical work and purification &ndas...
11 February 2013Uppsala, Sweden, 1st February, 2013 Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, has announced the launch of an updat...
04 September 2012Uppsala, Sweden, 4th September, 2012 Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces that Biotage is in late s...
15 June 2012Dr. Sunil Rana, product manager at Biotage, explains the advanced features for flash chromatography in the new Isolera™ Spektra release. Watch the free webinar recording here >
26 April 201223 April 2012 Product manager Bob Bickler introduces the new time-saving Isolera™ Spektra features for Isolera One, Four and LS Flash chromatography instruments.
18 October 2011Preparative FLASH chromatography expedites the production of clinical trial compounds. Tobi Williams is an application chemist specializing in pilot and production purification at Biotag...
16 August 2010Charlotte, North Carolina, USA, August 16, 2010 – Biotage (STO: BIOT), a leading developer of tools and technology for medicinal and analytical chemistry, announces the introduction of the Is...
21 April 2010Uppsala, Sweden, April 20, 2010 – Biotage (STO: BIOT), a global leader in automated flash purification, tools and technology for medicinal and analytical chemistry, announced it has recently ...
23 February 2010Uppsala, Sweden, February 23, 2010 – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, announced the launch of enhanced gradient optimization (GO) te...
27 October 2008October 28, 2008 Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, announces the release of its new 2008–09 Synthesis and Purification Catalog. The ...
Part No. | Description | Product type | Pack Size | Price | ||
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DALT-2000-APCI-100V | Biotage Dalton 2000 Mass Detector APCI 100 VAC incl roughing pump | Instruments | 1 | Log in for price | |
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DALT-2000-APCI-115V | Biotage Dalton 2000 Mass Detector APCI 115 VAC incl roughing pump | Instruments | 1 | Log in for price | |
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DALT-2000-APCI-230V | Biotage Dalton 2000 Mass Detector APCI 230 VAC incl roughing pump | Instruments | 1 | Log in for price |
Part No. | Description | Product type | Pack Size | Price | ||
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415337SP | Column Holder Sfär 200g/350g | Accessories | 1 | Log in for price | |
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415338SP | Column Holder Sfär 5g/10g | Accessories | 1 | Log in for price | |
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415339SP | Column Holder Sfär 25g | Accessories | 1 | Log in for price | |
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415343SP | Column Holder Sfär 50g/100g | Accessories | 1 | Log in for price | |
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416427SP | Column Holder Sfär 5g w. clip | Accessories | 0 | Log in for price | |
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DALT-2000-NLINK | Biotage Dalton 2000 Nanolink | Accessories | 1 | Log in for price | |
IS-ESI-S01 | Electrospray Ionization (ESI) ion source | Accessories | 1 | Log in for price | ||
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UP-ASAP-SNL | Dalton 2000 ASAP kit, probe, capillary stand, APCI source port | Accessories | 1 | Log in for price |