A group of researchers at Kobe Gakuin University (Japan), in collaboration with Biotage have published a new peer‑reviewed study in RSC Advances demonstrating a practical, DMF‑free aqueous solid‑phase peptide synthesis (ASPPS) method compatible with commercially available microwave peptide synthesizers, resins and Fmoc-protected amino acids.
The approach uses nanoassemblies of standard Fmoc amino acids to enable fast, efficient peptide coupling in water, including the successful synthesis of a 31‑residue peptide (b-endorphin), the longest reported to date using an aqueous coupling strategy.
The work highlights a realistic pathway towards sustainable peptide synthesis without disrupting established Fmoc-SPPS workflows using the Biotage® Initiator+ system .
Nanoassembly-enabled aqueous solid-phase peptide synthesis (ASPPS): a practical DMF-free approach based on the Fmoc strategy - RSC Advances (RSC Publishing)