Flash chromatography method development has historically been done using TLC plates. While this technique works in normal phase (silica, amine-functionalized silica), differences in media properties between the TLC and flash column in reversed phase can provide different selectivity and not provide accurate method information. For reversed phase chromatography, TLC is quite limited and not very useful due to poor water wettability. An alternative approach is provided by scaling columns.
Literature number: PPS625