---
title: Automated analysis of a panel of fat soluble vitamins A, D, E and K in serum by SLE
description: This poster investigates extraction optimization strategies to provide a method for the simultaneous analysis of Vitamin A, D, E and K.
---

[Literature ](https://www.biotage.com/literature)

# [Automated analysis of a panel of fat soluble vitamins A, D, E and K in serum by SLE](https://www.biotage.com/literature/poster/automated-analysis-panel-fat-soluble-vitamins-a-d-e-k-serum-sle)

 Written by [Biotage](https://www.biotage.com/literature/author/biotage) | Feb 24, 2026 2:41:21 PM

Simultaneous fat-soluble vitamin testing can be challenging for a variety of reasons: solubility limitations of the analytes, high levels of protein binding and wide variation of concentration ranges from one vitamin to another. This poster investigates extraction optimization strategies to provide a method for the simultaneous analysis of Vitamin A (Retinol and Beta Carotene), Vitamin D (25-OH D2 and D3), Vitamin E (Alpha Tocopherol) and Vitamin K (Phylloquinone K1, and Menaquinone K2). Samples could be processed manually or on the automated Extrahera™ platform. 

Literature number: P233

[View full post](https://www.biotage.com/literature/poster/automated-analysis-panel-fat-soluble-vitamins-a-d-e-k-serum-sle)

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