Concentrations of Vitamin B1 (Thiamine Diphosphate, TDP) and B6 (Pyridoxal-5-Phosphate, PLP) in whole blood are measured clinically to measure various metabolic functions. Accurate concentrations of vitamins B1 and B6 are difficult to obtain due to ion suppression found in LC-MS/MS analysis. Phospholipids and proteins are a main cause of ion suppression. Currently, laboratories are struggling to create accurate, precise, and robust sample preparation methods focused on the removal of these interferences.
This study aims to provide a sample preparation method with accurate and precise concentrations of vitamin B1 and B6 through the removal of interfering phospholipids and proteins. This study further aids in accuracy and pre cision through the use of automation.
Literature Number: P246