Literature

ISOLUTE® EPH SPE cartridges for the fractionation of EPH from soil or water using both automated and manual procedures

Written by Biotage | Dec 10, 2025 2:22:36 PM

This technical note provides procedures for both the automated and manual fractionation of EPH in soil or water extracts. Both procedures are described on page 2. Chromatograms illustrate the ability of ISOLUTE® EPH to provide efficient fractionation of hexane or pentane soil extracts into aliphatic and aromatic fractions (see Appendix).
ISOLUTE® EPH cartridges have been developed to fractionate pentane or hexane based soil or water extracts into aliphatic and aromatic hydrocarbon fractions (C8 – C40 aliphatic, C10 – C22 aromatics). The cartridges consist of a proprietary blend of silica/ alumina that has been optimised to achieve a clean fractionation of the aliphatic/aromatic EPH components. ISOLUTE® EPH cartridges have been designed to eliminate the common problem of aromatic breakthrough into the aliphatic fraction. 

Literature number: TN142.V.2