---
title: Evaluation of vitamin D extraction using PLD plate technology and method comparison using automated sample preparation
description: This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D.
---

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# [Evaluation of vitamin D extraction using PLD plate technology and method comparison using automated sample preparation](https://www.biotage.com/literature/poster/evaluation-vitamin-d-extraction-pld-method-comparison-automated-sample-preparation)

 Written by [Biotage](https://www.biotage.com/literature/author/biotage) | Feb 24, 2026 3:26:50 PM

Vitamin D deficiency can result in various health issues such as osteoporosis, liver and kidney problems and is associated with increased risk of cancers and multiple sclerosis. From this standpoint vitamin D analysis has extremely important clinical relevance. Many sample preparation approaches to the extraction of 25-hydroxy vitamin D have been employed prior to LC-MS/MS analysis. Simple protein precipitation, supported liquid extraction and complex SPE methodology are all in routine use. This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D. The extraction protocol was ultimately transferred to an SPE automation platform and method performance versus manual processing was compared. 

Literature number: P126

[View full post](https://www.biotage.com/literature/poster/evaluation-vitamin-d-extraction-pld-method-comparison-automated-sample-preparation)

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