Small-scale protein purification is an efficient method for screening samples for protein expression, stability, and functionality as well scaling down process development workflows for drug candidates. This approach conserves samples by minimizing material usage and expediting purification through parallel high-throughput techniques. Common platforms that are well-suited for small-scale protein purification include spin columns, filter plates, magnetic beads, and now PhyTip® Columns. Different workflows have unique
purification requirements and challenges. For instance, in screening labs, the primary objective is to isolate substantial quantities of protein for downstream analysis, while in process development, the focus is on optimizing purification methods and refining protocols to ensure reproducibility and facilitate production scalability. As throughput increases, manual platforms often face challenges such as low protein yields, sample handling errors, equipment limitations, and strenuous hands-on time.
Literature number: P273