Clinical & Bioanalytical Sample Preparation

Catecholamines & Metabolites

Highlighted Applications

Robust Extraction of Plasma Catecholamines and Metanephrines

Optimized for clean-up of plasma samples containing a range of anticoagulants, giving robust performance for analysis to <10 pg/mL (<0.05 nmol/L) by LC-MS/MS analysis.

Automated Extraction of Urinary Catecholamines and Metanephrines

A robust, mixed-mode weak cation exchange SPE protocol for the extraction of catecholamine and metanephrine metabolites from urine prior to LC-MS/MS detection. Fully automatable for high throughput assays, the method can prepare 96 samples in 52 minutes.

Acidic Catecholamine Metabolites in Plasma (VMA, HVA, 5-HIAA)

A simple ‘load-wait-elute’ supported liquid extraction methodology for the acidic metabolites, for LC-MS/MS analysis in the range of 5-200 mg/mL.

Automated Extraction of Plasma ‘Cats & Mets’ using Microelution SPE

Microelution format methodology using mixed-mode weak cation exchange SPE, automated using the Biotage® Extrahera™ LV-200. This robust method can extract 96 samples in approximately 60 minutes.

This application is also available in 10 mg format.

Application Note Finder

Biotage offer a wide range of optimized application notes and our new Application Notes Finder is under construction. In the meantime, don't hesitate to contact us. Our team of application specialists is here to support you!

Recommended Products

ISOLUTE® SLE+

Supported Liquid Extraction plates and columns designed to provide high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple.

EVOLUTE® EXPRESS WCX

Eliminate conditioning and equilibration with EVOLUTE® EXPRESS load-wash-elute SPE. Robust, reliable SPE, giving ultra-pure extracts and high recoveries. Specifically developed for quantification by LC-MS/MS.

Biotage® Mikro SPE

Excellent sample clean up while enhancing manual or automated sample preparation workflow and delivering reproducible, high sensitivity analytical results. For elution volumes as low as 20 µL.

Positive Pressure Processing

Consistent, uniform flow of positive pressure to move both low and high viscosity liquids through plates and columns.

Extrahera™ LV-200

The automated sample prep workstation built to process low volume samples with the ease of manual sample prep and the digital integrity of a liquid handler, all in one small footprint.

96 Well Plate Evaporators

Designed for high throughput laboratories, to provide efficient solvent evaporation in microplate formats.

The revolution of microelution in solid phase extraction

Find out how microelution SPE works to increase analytical sensitivity without the need for evaporation.

Resources

Watch the Webinar

Perfecting Pcats – Developing a More Robust UHPLC-MS/MS Method for Plasma Catecholamines using Low Volume SPE.

Clinical Applications Compendium

Selection of clinical sample preparation applications from Biotage.

Avoiding Cross Talk 96-well Based Sample Preparation

Eliminate carryover between samples, and reduce the risk of false positive results.

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Make your life easier. Simplify your workflow

Automated sample preparation reduces errors, improves method robustness and frees up laboratory personnel for other tasks.

Automation

Biotage evaporation systems are designed for fast sample evaporation and minimizing sample transfer.

Evaporation & Concentration

Discover the full range of sample preparation products from Biotage.

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