--- title: Clinical & Bioanalytical Sample Preparation description: Biotage sample preparation applications and products for clinical and bioanalytical laboratories --- CLOSE × Clinical & Bioanalytical Sample Preparation CAT_ANA_APP_CLINICAL&BIOANALYTICAL_v3b # Clinical and Bioanalytical Sample Preparation Whether its vitamins, steroids & hormones or drug panels that you need to analyse, we can help you. Sample preparation to remove matrix components such as proteins, phospholipids and salts from your biological fluid samples can improve analyte sensitivity, method robustness and improve the quality of analytical data, as well as reducing instrument downtime. Discover Biotage smart sample preparation solutions and find lots of useful resources for clinical applications below. ![biomark002.366 - Clinical Testing](https://www.biotage.com/hs-fs/hubfs/ANALYTICAL/IMAGES/WEBINAR%20IMAGES/biomark002.366%20-%20Clinical%20Testing.jpg?width=780&name=biomark002.366%20-%20Clinical%20Testing.jpg) #### [Clinical applications compendium](https://www.biotage.com/documents/clinical-applications-compendium?hsLang=en) #### [Webinar: Considerations for minimizing matrix effects and maximising recovery](https://www.biotage.com/considerations-for-minimizing-matrix-effects-and-maximising-recovery-for-clinical-panels-large-and-small-on-demand-webinar) #### [The complete guide for bioanalytical sample preparation](https://www.biotage.com/biofluids-sample-prep) #### [The benefits of automating sample preparation](https://www.biotage.com/automating-sample-preparation) Catecholamines & Metabolites Vitamins Steroids & Hormones Pain Management Panel Therapeutic Drug Research ## Catecholamines & Metabolites APPLICATION NOTE [ Robust Extraction of Plasma Catecholamines and Metanephrines ](https://www.biotage.com/documents/extraction-catecholamines-metanephrines-human-plasma-evolute-express-wcx?hsLang=en) Optimized for clean-up of plasma samples containing a range of anticoagulants, giving robust performance for analysis to <10 pg/mL (<0.05 nmol/L) by LC-MS/MS analysis. APPLICATION NOTE [ Automated Extraction of Urinary Catecholamines and Metanephrines ](https://www.biotage.com/documents/extraction-urinary-catecholamines-metanephrines-evolute-express-wcx-spe?hsLang=en) A robust, mixed-mode weak cation exchange SPE protocol for the extraction of catecholamine and metanephrine metabolites from urine prior to LC-MS/MS detection. Fully automatable  for high throughput assays, the method can prepare 96 samples in 52 minutes.     APPLICATION NOTE [ Automated Extraction of Plasma ‘Cats & Mets’ using Microelution SPE ](https://www.biotage.com/documents/automated-extraction-catecholamines-metanephrines-human-plasma-biotage-extrahera-lv-200-microelution-spe?hsLang=en) Microelution format methodology using mixed-mode weak cation exchange SPE, automated using the Biotage® Extrahera™ LV-200. This robust method can extract 96 samples in approximately 60 minutes. WEBINAR [ Perfecting pCats – Developing a More Robust UHPLC-MS/MS Method for Plasma Catecholamines using Low Volume SPE ](https://www.biotage.com/developing-a-more-robust-uhplc-ms-ms-method-for-plasma-catecholamines-using-low-volume-spe-on-demand-webinar) More documents ## Vitamins WEBINAR [ Trials and Tribulations of Fat Soluble Vitamin Analysis by UHPLC-MS/MS ](https://www.biotage.com/trials-and-tribulations-of-fat-soluble-vitamin-analysis-by-uhplc-ms-ms-on-demand-webinar) BLOG [ Why just measure Vitamin D when you can monitor Vitamins A, E and K as well? ](https://www.biotage.com/blog/why-just-measure-vitamin-d-when-you-can-monitor-vitamins-a-e-and-k-as-well) This blog post helps developing a method for the extraction of a panel of vitamins A, E and K. APPLICATION NOTE [ Fat-Soluble Vitamins ](https://www.biotage.com/documents/extraction-fat-soluble-vitamins-human-serum-isolute-sle?hsLang=en) Simultaneous extraction of fat soluble vitamins (A, D, E and K) from serum presents a number of challenges, from the diversity of molecular structures and properties, to the wide variation in relevant clinical concentrations. Biotage specialists developed a method based on the Supported Liquid Extraction technique that effectively addresses these challenges. APPLICATION NOTE [ Extraction of Vitamin B7, Biotin ](https://www.biotage.com/documents/extraction-vitamin-b7-serum-evolute-express-abn?hsLang=en) Vitamin B7 (Biotin) is a water-soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids.  More documents ## Steroids & Hormones APPLICATION NOTE [ Simple, Sensitive Extraction of 19 Human Steroids from Serum ](https://www.biotage.com/documents/extraction-comprehensive-steroid-panel-human-serum-isolute-sle?hsLang=en) Extraction method of a comprehensive panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis.   APPLICATION NOTE [ Automated Extraction of Urinary Catecholamines and Metanephrines ](https://www.biotage.com/documents/extraction-urinary-catecholamines-metanephrines-evolute-express-wcx-spe?hsLang=en) A robust, mixed-mode weak cation exchange SPE protocol for the extraction of catecholamine and metanephrine metabolites from urine prior to LC-MS/MS detection. Fully automatable  for high throughput assays, the method can prepare 96 samples in 52 minutes.   APPLICATION NOTE [ Simultaneous Extraction of Aldosterone and Angiotensin for PRA Analysis ](https://www.biotage.com/documents/simultaneous-extraction-aldosterone-angiotensin-i-ii-human-plasma-evolute-express-abn?hsLang=en) Plasma renin activity (PRA) determination is used in the diagnosis of primary aldosteronism, an important cause of high blood pressure.  This application note describes the simultaneous extraction of aldosterone at low (< 25pg/mL) concentration and angiotensin I and II from plasma, with the potential to improve throughput and reduce time and costs. APPLICATION NOTE [ Thyroid Hormones (T3, rT3 and T4) in Serum ](https://www.biotage.com/documents/extraction-thyroid-hormones-t3-rt3-t4-serum-evolute-express-ax-plates?hsLang=en) This extraction method using EVOLUTE® EXPRESS AX 96-well plates provides extremely clean, phospholipid and pigment free extracts which help maintain analytical system sensitivity.   More documents ## Pain Management Panel BLOG [ How to extract and analyze for ever-present contaminants of interest? ](https://www.biotage.com/blog/how-to-extract-and-analyze-for-ever-present-contaminants-of-interest) It happens to all of us. We're getting a new method developed and validated and then it comes time to run our negative urines. And everything comes up as positive! There are peaks for our analytes of interest in every urine that we run! How is that possible? WHITE PAPER [ Current Methodologies for Drugs of Abuse Urine Testing ](https://www.biotage.com/documents/current-methodologies-doa-urine-testing-white-paper?hsLang=en) This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels. Originally published in 2017, the white paper has now been updated to include details of additional hydrolysis enzymes and optimum hydrolysis conditions. APPLICATION NOTE [ In-well Hydrolysis: Streamline Your Sample Handling ](https://www.biotage.com/documents/in-well-hydrolysis-spe-method-evolute-hydro-cx-96-licit-illicit-doa-urine?hsLang=en) Save time and eliminate sample transfer steps, reducing the risk of errors and improving method robustness.  This application note describes the extraction and clean up of 96 licit and illicit drugs using in-well hydrolysis of the urine sample. APPLICATION NOTE [ Urine Hydrolysis: Does it matter how you do it? ](https://www.biotage.com/documents/automated-approach-urine-sample-preparation-room-temperature-enzymatic-hydrolysis?hsLang=en) Urine hydrolysis is performed to eliminate glucuronide drug metabolites before analysis, and it is key part of the sample preparation process. But it can involve extra sample transfer steps, and adds time to the sample preparation process. Streamline your workflow with integral in-well hydrolysis. WEBINAR [ Advances in Sample Prep Using In-well Hydrolysis ](https://www.biotage.com/advances-in-sample-prep-using-in-well-hydrolysis-on-demand-webinar) More documents ## Therapeutic Drug Research WEBINAR [ A New, Streamlined Approach to Multiple Immunosuppressant Sample Preparation for LCMS analysis ](https://www.biotage.com/a-new-streamlined-approach-to-multiple-immunosuppressant-sample-preparation-for-lcms-analysis-on-demand-webinar) APPLICATION NOTE [ Therapeutic Drugs- Mycophenolic Acid in Plasma ](https://www.biotage.com/documents/sample-preparation-method-determination-mycophenolic-acid-plasma-isolute-sle?hsLang=en) APPLICATION NOTE [ Therapeutic Drugs - Voriconazole in Plasma ](https://www.biotage.com/documents/sample-preparation-method-determination-voriconazole-plasma-isolute-sle?hsLang=en) APPLICATION NOTE [ Therapeutic Drugs - Axitinib in Plasma ](https://www.biotage.com/documents/sample-preparation-method-for-determination-of-axitinib-in-plasma-using-isolute-sle?hsLang=en) APPLICATION NOTE [ Therapeutic Drugs - GS-441524 in Plasma ](https://www.biotage.com/documents/sample-preparation-method-for-determination-of-gs-441524-in-plasma-using-isolute-env-spe-columns?hsLang=en) APPLICATION NOTE [ Therapeutic Peptides - Desmopressin in Serum ](https://www.biotage.com/documents/extraction-desmopressin-serum-evolute-express-wcx?hsLang=en) APPLICATION NOTE [ Therapeutic Peptides - Teicoplanin in Plasma ](https://www.biotage.com/documents/extraction-teicoplanin-plasma-evolute-express-abn?hsLang=en) APPLICATION NOTE [ Therapeutic Peptides - Oxytocin and Vasopressin in Serum ](https://www.biotage.com/documents/extraction-oxytocin-vasopressin-serum-evolute-express-abn?hsLang=en) More documents × ## Your Cart Request a Quote Checkout Recommended Products Supported Liquid Extraction plates and columns designed to provide high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple. [ ISOLUTE® SLE+](https://www.biotage.com/products/isolute-sle-supported-liquid-extraction-cartridges-plates) Eliminate conditioning and equilibration with EVOLUTE® EXPRESS load-wash-elute SPE. Robust, reliable SPE, giving ultra-pure extracts and high recoveries. Specifically developed for quantification by LC-MS/MS. [ EVOLUTE® EXPRESS](https://www.biotage.com/polymeric-solid-phase-extraction) Excellent sample clean up while enhancing manual or automated sample preparation workflow and delivering reproducible, high sensitivity analytical results. For elution volumes as low as 20 µL. Biotage® Mikro Powerful, user friendly automation systems for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD) and Protein Precipitation (PPT) based methods, in plate or column formats. [ Biotage® Extrahera™](https://www.biotage.com/products/solid-phase-extraction-systems) Consistent, uniform flow of positive pressure to move both low and high viscosity liquids through plates and columns. [ Biotage® PRESSURE+](https://www.biotage.com/products/pressure-plus-positive-pressure-manifold) Designed for high throughput laboratories,  to provide efficient solvent evaporation in microplate formats. [ TurboVap® 96 Dual](https://www.biotage.com/products/turbovap-96-dual-evaporator) ### About Biotage Biotage is a global supplier and solutions partner to a wide range of customers within drug discovery and development and analytical testing. With strong expertise in separation and purification technologies, and a focus on leveraging intelligent workflow solutions, we aim to efficiently support the advancement and protection of human health. 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