Clinical & Bioanalytical Sample Preparation

Steroids & Hormones

Highlighted Applications

Simple, Sensitive Extraction of 19 Human Steroids from Serum

Extraction method of a comprehensive panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis.

Automated Extraction of Urinary Catecholamines and Metanephrines

A robust, mixed-mode weak cation exchange SPE protocol for the extraction of catecholamine and metanephrine metabolites from urine prior to LC-MS/MS detection. Fully automatable for high throughput assays, the method can prepare 96 samples in 52 minutes.

Simultaneous Extraction of Aldosterone and Angiotensin for PRA Analysis

Plasma renin activity (PRA) determination is used in the diagnosis of primary aldosteronism, an important cause of high blood pressure. This application note describes the simultaneous extraction of aldosterone at low (< 25pg/mL) concentration and angiotensin I and II from plasma, with the potential to improve throughput and reduce time and costs.

Thyroid Hormones (T3, rT3 and T4) in Serum

This extraction method using EVOLUTE® EXPRESS AX 96-well plates provides extremely clean, phospholipid and pigment free extracts which help maintain analytical system sensitivity.

Application Note Finder

Biotage offer a wide range of optimized application notes and our new Application Notes Finder is under construction. In the meantime, don't hesitate to contact us. Our team of application specialists is here to support you!

Recommended Products

ISOLUTE® SLE+

Supported Liquid Extraction plates and columns designed to provide high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple.

EVOLUTE® EXPRESS WCX

Eliminate conditioning and equilibration with EVOLUTE® EXPRESS load-wash-elute SPE. Robust, reliable SPE, giving ultra-pure extracts and high recoveries. Specifically developed for quantification by LC-MS/MS.

Biotage® Mikro SPE

Excellent sample clean up while enhancing manual or automated sample preparation workflow and delivering reproducible, high sensitivity analytical results. For elution volumes as low as 20 µL.

Positive Pressure Processing

Consistent, uniform flow of positive pressure to move both low and high viscosity liquids through plates and columns.

Extrahera™

Powerful, user friendly automation system for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD) and Protein Precipitation (PPT) based methods, in plate or column formats.

96 Well Plate Evaporators

Designed for high throughput laboratories, to provide efficient solvent evaporation in microplate formats.

The revolution of microelution in solid phase extraction

Find out how microelution SPE works to increase analytical sensitivity without the need for evaporation.

Resources

Clinical Applications Compendium

Selection of clinical sample preparation applications from Biotage.

Get Started with Supported Liquid Extraction

Method development chart, tips and tricks and useful insights to optimize your sample prep.

Avoiding Cross Talk 96-well Based Sample Preparation

Eliminate carryover between samples, and reduce the risk of false positive results.

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Make your life easier. Simplify your workflow

Automated sample preparation reduces errors, improves method robustness and frees up laboratory personnel for other tasks.

Automation

Biotage evaporation systems are designed for fast sample evaporation and minimizing sample transfer.

Evaporation & Concentration

Discover the full range of sample preparation products from Biotage.

All Products for Sample Preparation