Highlighted Applications

Simultaneous extraction of fat soluble vitamins (A, D, E and K) from serum presents a number of challenges, from the diversity of molecular structures and properties, to the wide variation in relevant clinical concentrations. Biotage specialists developed a method based on the Supported Liquid Extraction technique that effectively addresses these challenges.

Vitamin B7 (Biotin) is a water-soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. 

Over the last 20 years, renewed scientific interest in the functions of Vitamin D in the body have required the development of sensitive methods for extraction and analysis of its various metabolites. Our specialists have developed the following application notes to deliver high analyte recoveries and clean extracts from serum.

Protocol for the extraction of 1,25 di-OH Vitamin D metabolites from serum using supported liquid extraction  prior to LC-MS/MS detection. 

Simple, effective protocol for the extraction of 25-hydroxy vitamin D from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.

Biotage offer a wide range of optimized application notes and our new Application Notes Finder is under construction. In the meantime, don't hesitate to contact us. Our team of application specialists is here to support you!