Purifying peptides from crude synthetic mixtures can be extremely challenging. The Biotage® Resolux™ range of high resolution reversed-phase HPLC columns and guard cartridges with different packing pore size, column bonded phases and diameters are available to optimize separation of any peptide. All Resolux products are available as analytical, semi-preparative and preparative HPLC columns, ranging from 2.1 mm to 20 mm diameter for predictable scaleup. HPLC guard cartridges for in-line front-end protection of all HPLC columns are also available. Optimized phase bonding and column packing techniques ensure unsurpassed performance and column-tocolumn reproducibility and reliability.
LysM C-terminal separation on Resolux 120
The LysM domain is involved in the regulation of the interaction between plants and rhizobial bacteria to promote plant growth. The C-terminal of the LysM domain is difficult to synthesize, consequently leading to several amino acid deletions during solid phase peptide synthesis. The chromatogram shows a crude synthetic sample of the C-terminal sequence of the LysM domain efficiently being separated from a peptide which differs in only one amino acid.
Heptad repeat sequence separation on Resolux 200
The heptad repeat sequences are amphipathic peptides which are involved in a-helical coiled-coils and have been used for the De novo design of protein structures. Consequently, they have been a subject for extensive research. The chromatogram shows a crude synthetic sample of a 31-mer heptad repeat peptide efficiently separated from side products formed during solid phase peptide synthesis.
Peptide chemists typically have a broad range of requirements for their analytical chromatography. Biotage offers many dimensions of each Resolux product line. Peptide chemists can select between three different internal diameters; 2.1, 3.0 and 4.6 mm, where each is available in three different lengths; 100, 150 and 250 mm.
When peptide chemists need to collect samples of their isolated compounds, larger columns with more capacity are needed. Biotage offers Resolux as semi-preparative and preparative columns with internal diameters of 10 mm and 20 mm. Each of these columns are available in lengths of 100, 150 and 250 mm.
Applying guard cartridges as in-line front end protection of HPLC columns can significantly increase the life of the HPLC column. Biotage offers Resolux guard cartridge at analytical scale with 4.5 µm particles, as well as semi-prep and preparative scale with either 4,5 or 10 µm particles.
- High resolution reversed-phase HPLC columns and guard cartridges
- Analytical, semi-preparative and preparative columns in 3 different lengths (100 mm, 150 mm, 250 mm)
- C4, C8 and C18 bonded phases for improved selectivity
- Separation of peptides differing by as little as one amino acid
- Three different pore sizes; 120 Å, 200 Å, 300 Å
- Economical while delivering exceptional performance and unrivaled efficiency in demanding situations
- Column: Resolux 120, C18, 4,6 x 150 mm
- Eluent A: H2O; 0.1% TFA.
- Eluent B: CH3CN; 0.1% TFA
- Elution gradient: Eluent B 10-50% in 32 min.
- Flow rate: 1 mL/min
- Sample: Dissolved in the mobile phase
- Detection: UV 215 nm