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Extracting both acidic and basic analytes in a single SLE procedure
Clinical, Forensic and Doping Testing
This blog provides two strategies that can be used to extract both acids and bases in the same SLE procedure.
Does media choice matter for peptide library purification using solid phase extraction?
Small Molecules and Synthetic Therapeutics
Solid phase extraction is emerging as a strategy for peptide library clean up in parallel. While reversed phase is a robust media choice, ...
What is the difference between solid vs. liquid particles in ELSD signal?
Small Molecules and Synthetic Therapeutics
ELSD is useful for finding molecules that have no chromophore during flash purification. Here we show how signal intensity can vary between ...
How does the make-up solvent impact Evaporative Light-scattering detection?
Small Molecules and Synthetic Therapeutics
This post describes how ELSD make-up solvent choice can impact detected peak sensitivity.
Purifying natural product extracts? Why you should use ELSD with UV detection
Small Molecules and Synthetic Therapeutics
This post highlights the benefits achievable when using both UV and ELS detection.
Why you should add an ELSD to your flash chromatography system
Small Molecules and Synthetic Therapeutics
This post shows an example of a reaction mix purification requiring ELS detection.
Can Coupling Reagents Cause Premature Cleavage from Cl-Trityl Resin?
Small Molecules and Synthetic Therapeutics
DIC/Oxyma coupling conditions are thought to be acidic enough to prematurely cleave peptide from chloro-trityl resins. Here we show how ...
How to choose dry load media
Small Molecules and Synthetic Therapeutics
This post highlights the benefits provided by various dry load media.
Why do I have waves in my chromatogram?
Small Molecules and Synthetic Therapeutics
This is a troubleshooting post discussing the causes and remedies for wavy chromatographic traces.
Can organic solvent choice impact reversed phase flash chromatography separations?
Small Molecules and Synthetic Therapeutics
This post describes how organic solvent choice impacts reversed phase separations.
