AAV PhyTip® column
Protein purification column
Automated purification of adeno-associated viruses using CaptureSelect™ AAVX resin for high selectivity, 60-80% recovery, and fast, consistent results.
Adeno-associated virus (AAV) is a non-enveloped virus commonly engineered to deliver DNA to target cells. Its single-stranded DNA genome (~4.7 kb) makes it a preferred vector for gene therapy, enabling delivery to diverse cell types including muscle, vascular endothelial, and neuronal cells.
In downstream processing, affinity chromatography effectively removes impurities such as plasmid DNA and host cell RNA/DNA, followed by anion exchange polishing to eliminate empty capsids.
PhyTip® columns with CaptureSelect™ AAVX affinity matrix are designed for high selectivity and capacity in the purification of both naturally occurring and synthetic AAV serotypes. The CaptureSelect™ AAVX resin binds efficiently to AAV1–AAV9, AAVrh10, and a range of synthetic variants used in gene therapy applications.
Using dual flow chromatography, PhyTip® columns pump samples back and forth across the packed resin bed to maximize binding. Packed with a high-capacity AAV affinity resin, they routinely deliver purified protein samples with 60–80% recovery.
AAV PhyTip® columns are compatible with most 8-, 12- and 96-channel automated liquid handling systems, providing fast and consistent purification across multiple formats.
CaptureSelect™ is a trademark of Thermo Fisher Scientific.
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Biomolecule purification with PhyTip® columns
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Product note
POROS CaptureSelect™ AAVX PhyTip® columns
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White paper
Accelerating high throughput with PhyTip® columns
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White paper
PhyTip® columns versus other common technologies: A comparative analysis of protein purification yields
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White paper
PhyTip® columns versus other common technologies: a comparative analysis of protein purification efficiency and yield consistency
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