The UV absorption spectrum of some solvents
overlaps with the product they dissolve, meaning
that fraction collection processes cannot distinguish
between solvent and product. Luckily, there is
technology that solves this problem.
Tags: Application Notes
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
» Isocratic separations are easiest to develop, but gradient separations are
» Software in the Isolera helps with conversion of an isocratic separation to
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
Tags: Manuals & User Guides
For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed.
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica
because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or
THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration.
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
The adapter kit contains a pre-assembled stand, spacer and
connecting tubing; everything required to allow the use of
SNAP 340g cartridges on flash purification systems that utilize
Luer tubing connections such as, Isco Companion and Analogix
Tags: English, Product Notes
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Isolera™ Prime is a value based system which recommends appropriate cartridges and calculates the best solvent conditions and gradients for your purifications. It has solvent pumps, automated fraction collection as standard and delivers much more than just the basics. Solvent use is minimized and advanced compound detection makes sure that your purified compound is delivered at the end of the run. We belive in providing value, so Isolera Prime systems embrace ACI™, a revolutionary advancement, converting regular flash from simple purification to the fastest, greenest, and most economical way to reliably isolate pure compounds for organic synthesis. ACI offerred as standard on Isolera Prime.
Isolera™ Dalton is the most advanced and innovative flash purification system available, designed by people who understand flash chromatography for the chemists who need it.
"Pure convenience! Just pure convenience! It lets us perform purification and analysis at the same time. It gives us tremendous power to isolate unknown compounds.”
- Hiroki Nishii, Chugai Pharmaceutical’s Kamakura Research Laboratories
|Part No.||Description||Product type||Pack Size||Price|
|120-1110||FLASH LUER LOCK TO LUER ADAPTORS PK/25||Accessories||25||Log in for price|
|411069||FlashMaster II Holder||Accessories||1||Log in for price|
|412358||XL Fitting, Female Luer, Assembly - Isolera LS||Accessories||1||Log in for price|
|412537||Male Luer outlet fitting for SNAP 750g and 1500g cartridges||Accessories||1||Log in for price|
|413027||3-way large bore stainless steel injection valve for Isolera systems||Accessories||1||Log in for price|
|413092||Biotage ZIP 5g/10g cartridge holder||Accessories||1||Log in for price|
|413302||Biotage ZIP 30g cartridge holder||Accessories||1||Log in for price|
|413303||biotage ZIP 45g cartridge holder||Accessories||1||Log in for price|
|413304||Biotage ZIP 80g cartridge holder||Accessories||1||Log in for price|
|413305||Biotage ZIP 120g cartridge holder||Accessories||1||Log in for price|