Item added to cart
Shopping Cart (Login required)
Sample Preparation Horizon Food & Environmental Products Organic Synthesis Peptide Chemistry Flash Purification Work-up Products Evaporation & Concentration Industrial & Large Scale Products Biomolecules Service & Support

Dry Load Vessels

Improve Purification Results

DLV-025
DLV-500 DLV-025

Biotage® columns are designed with the ability to dry load a sample within the cartridge.  Sometimes, the amount of adsorbent required exceeds the cartridge’s dry load capacity, or in the case of some formats, the dry loading needs to be external. When that situation arises, the Biotage DLV systems provide a convenient in-line solution.

 

The solution for hard-to-dissolve crude samples

One of the most common flash purification challenges is dealing with hard-to-dissolve crude samples. Because excess polar solvents cause poor chromatographic results, other sample load options are needed. A commonly used solution is dry loading, which involves dissolving the sample in a suitable polar solvent, mixing the solution with an inert, clean, dry adsorbent such as silica or diatomaceous earth, and drying the slurry. By drying the mixture, the polar solvent is removed and will have no impact on the purification. The dried sample is then loaded into an empty vessel and inserted infront of the purification cartridge.  Dry load vessels are available for use with all Biotage cartridges.

Biotage® Dry Load Vessels (DLV) are available in four size ranges to address the dry load needs at any purification scale. 

  • DVL-010 - can be used with up to 10g of solid load sorbent
  • DLV-025 - can be used with up to 25 g of solid load sorbent
  • DLV-050 - can be used with up to 100g of solid load sorbent
  • DLV-500 - can be used with up to 500g of solid load sorbent

The DLV plungers above attach directly to the top of the Biotage® Sfär column (DLV-010, DLV-035, DLV-050) and uses a Luer-Lok to secure good operation. DLV columns exist in both empty and pre-packed versions. Prefilled vessels are available in silica and HM-N (diatomaceous earth). 

For the Isolera™ Spektra LS the DLV-500 (up to 500 g of solid load sorbent) is recommended. Empty 500g capacity vessels are also available.

Safe and simple to use

A common flash purification challeng is hard-to-dissolve crude samples. Dry loading is an effective option, which involves dissolving the sample, mixing with an adsorbent and drying the slurry. The dried sample is then loaded into an empty vessel and inserted ahead of the purification cartridge.

 

Dry loading for Flash 75 and 150 cartridges

Flash 75 and 150 systems use a Biotage® SIM (Sample Introduction Module) for sample dry loading. SIMs are stainless steel vessels which are placed between the solvent reservoir and the cartridge. The SIM can be filled with preabsorbed compound or viscous liquid samples. The absorbed compounds are then eluted with solvent and separated in the flash cartridge. Viscous liquid samples are moved into the Flash cartridge using compressed air. Several sizes are available to meet differing load requirements including SIM 100, SIM 200, SIM 500, SIM-1000 and SIM-2000.

Advantages

  • Improved sample separation
  • Higher loading capacity
  • Increased fraction purity

CONSUMABLES

Part No. Description Product type Pack Size Price
415325SP O-RING, Ø 22x3 PERFLOUR Consumables 2 Log in for price
415335SP O-RING, Ø 32x3 PERFLOUR Consumables 2 Log in for price
DLV-0000-0025 DLV-0000-0010 Biotage® Sfär DLV ­ Empty 10g Column w. frit, 20/cs Consumables 20 Log in for price
DLV-0000-0025 DLV-0000-0025 Biotage® Sfär DLV ­ Empty 25 g Column w. frit, 20/cs Consumables 20 Log in for price
DLV-0000-0025 DLV-0000-0050 Biotage® Sfär DLV ­ Empty 50 g Column w. frit, 20/cs Consumables 20 Log in for price
DLV-0000-0025 DLV-0000-0100 Biotage® Sfär DLV ­ Empty 100 g Column w. frit, 10/cs Consumables 10 Log in for price
DLV-0445-0010 Biotage® Sfär Silica DLV ­ Column with 5 g Silica, 10/cs Consumables 10 Log in for price
DLV-0445-0025 Biotage® Sfär Silica DLV ­ Column with 10 g Silica, 10/cs Consumables 10 Log in for price
DLV-0445-0050 Biotage® Sfär Silica DLV ­ Column with 25 g Silica, 5/cs Consumables 5 Log in for price
DLV-0825-0010 Biotage® Sfär HMN-R DLV ­ Column with 5 g HMN-R, 10/cs Consumables 10 Log in for price
DLV-0825-0025 DLV-0825-0025 Biotage® Sfär HMN-R DLV ­ Column with 10 g HMN-R, 10/cs Consumables 10 Log in for price
DLV-0825-0050 Biotage® Sfär HMN-R DLV ­ Column with 25 g HMN-R, 5/cs Consumables 5 Log in for price
DLV-505 Empty dry load cartridges and frits, 4/cs Consumables 4 Log in for price

ACCESSORIES

Part No. Description Product type Pack Size Price
DLV-010 DLV-010 Biotage® Sfär DLV ­ Plunger for 10 g Column Accessories 1 Log in for price
DLV-025 DLV-025 Biotage® Sfär DLV ­ Plunger for 25 g Column Accessories 1 Log in for price
DLV-050 DLV-050 Biotage® Sfär DLV ­ Plunger for 50/100 g Column Accessories 1 Log in for price
DLV-500 DLV-500 Dry Load Vessel for Isolera LS, 1/cs Accessories 1 Log in for price

SPARE PARTS

Part No. Description Product type Pack Size Price
412626 O-ring for DLV-500, Chemraz Spare parts 1 Log in for price
415316SP Biotage® Sfär DLV 10 O-RING, 2 pcs Spare parts 2 Log in for price