The solution for hard-to-dissolve crude samples
One of the most common flash purification challenges is dealing with hard-to-dissolve crude samples. Because excess polar solvents cause poor chromatographic results, other sample load options are needed. A commonly used solution is dry loading, which involves dissolving the sample in a suitable polar solvent, mixing the solution with an inert, clean, dry adsorbent such as silica or diatomaceous earth, and drying the slurry. By drying the mixture, the polar solvent is removed and will have no impact on the purification. The dried sample is then loaded into an empty vessel and inserted infront of the purification cartridge. Dry load vessels are available for use with all Biotage cartridges.
Biotage® Dry Load Vessels (DLV) are available in four size ranges to address the dry load needs at any purification scale.
- DVL-010 - can be used with up to 10g of solid load sorbent
- DLV-025 - can be used with up to 25 g of solid load sorbent
- DLV-050 - can be used with up to 100g of solid load sorbent
- DLV-500 - can be used with up to 500g of solid load sorbent
The DLV plungers above attach directly to the top of the Biotage® Sfär column (DLV-010, DLV-035, DLV-050) and uses a Luer-Lok to secure good operation. DLV columns exist in both empty and pre-packed versions. Prefilled vessels are available in silica and HM-N (diatomaceous earth).
For the Isolera™ Spektra LS the DLV-500 (up to 500 g of solid load sorbent) is recommended. Empty 500g capacity vessels are also available.
Safe and simple to use
A common flash purification challeng is hard-to-dissolve crude samples. Dry loading is an effective option, which involves dissolving the sample, mixing with an adsorbent and drying the slurry. The dried sample is then loaded into an empty vessel and inserted ahead of the purification cartridge.
Dry loading for Flash 75 and 150 cartridges
Flash 75 and 150 systems use a Biotage® SIM (Sample Introduction Module) for sample dry loading. SIMs are stainless steel vessels which are placed between the solvent reservoir and the cartridge. The SIM can be filled with preabsorbed compound or viscous liquid samples. The absorbed compounds are then eluted with solvent and separated in the flash cartridge. Viscous liquid samples are moved into the Flash cartridge using compressed air. Several sizes are available to meet differing load requirements including SIM 100, SIM 200, SIM 500, SIM-1000 and SIM-2000.
- Improved sample separation
- Higher loading capacity
- Increased fraction purity