Protein A PhyTip® column
Protein purification column
Automated and fast purification and concentration of IgG antibodies with 70–90% recovery.
Protein A is the most widely used affinity media for antibody purification. Both protein G and protein A bind to the Fc region of IgG antibodies, with varying selectivity for different IgG subtypes. Our Protein A PhyTip® columns efficiently extract functional IgG antibodies with high purity, even from complex backgrounds, thanks to their strong binding affinity and high capacity.
PhyTip® columns use dual flow chromatography technology, pumping samples back and forth across the packed resin to maximize binding. Efficient capture minimizes protein loss, with 70–90% recovery of lgGs, and a full 96-well plate can be purified in just 15 minutes.
The columns come with dual flow chromatography protocols, validated with real protein samples and fully transferable to any automated liquid handling system. Our buffer kits contain all the buffers needed for the purification protocol.
Resources
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Download
Product Note
Protein A PhyTip® columns
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Download
Technical note
Maximize antibody recovery with PrismA PhyTip® columns
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Read article
White paper
PhyTip® columns versus other common technologies: a comparative analysis of protein purification yields
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Read article
White paper
PhyTip® columns versus other common technologies: a comparative analysis of protein purification efficiency and yield consistency
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White paper
Accelerating high throughput with PhyTip® columns
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Read article
Application note
High Performance Immunoprecipitation (HPIP), Direct IP method
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Read article
Application note
High Performance Immunoprecipitation (HPIP), Indirect method
Ordering information
Part no.
Name
Pack size
Price
Part no.
PTX-91-10-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Dynamic Devices
Pack size
96
Price
826 USD
Part no.
PTB-91-16-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman
Pack size
96
Price
3229 USD
Part no.
PTX-91-16-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Dynamic Devices
Pack size
96
Price
3240 USD
Part no.
PTH-91-16-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton
Pack size
96
Price
3240 USD
Part no.
PTR-91-16-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, ME/MEA/Rainin
Pack size
96
Price
3229 USD
Part no.
PTT-91-16-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan
Pack size
96
Price
3240 USD
Part no.
PTB-91-20-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman
Pack size
96
Price
993 USD
Part no.
PTX-91-20-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Dynamic Devices
Pack size
96
Price
993 USD
Part no.
PTH-91-20-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton
Pack size
96
Price
982 USD
Part no.
PTR-91-20-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, ME/MEA/Rainin
Pack size
96
Price
982 USD
Part no.
PTB-91-40-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Beckman
Pack size
96
Price
1463 USD
Part no.
PTH-91-40-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton
Pack size
96
Price
1463 USD
Part no.
PTR-91-40-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, ME/MEA/Rainin
Pack size
96
Price
1463 USD
Part no.
PTT-91-40-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan
Pack size
96
Price
1463 USD
Part no.
PTB-91-80-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman
Pack size
96
Price
2299 USD
Part no.
PTX-91-80-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Dynamic Devices
Pack size
96
Price
2268 USD
Part no.
PTH-91-80-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton
Pack size
96
Price
2299 USD
Part no.
PTR-91-80-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, ME/MEA/Rainin
Pack size
96
Price
2268 USD
Part no.
PTT-91-80-55
Name
MabSelect PrismA™ PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan
Pack size
96
Price
2299 USD
Part no.
PTT-95-16-55
Name
MabSelect PrismA™ PhyTip® columns (500 µL volume) containing 160 µL affinity media per column, Tecan
Pack size
96
Price
3240 USD
Part no.
PTT-95-40-55
Name
MabSelect PrismA™ PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan
Pack size
96
Price
1463 USD
Part no.
PTT-95-80-55
Name
MabSelect PrismA™ PhyTip® columns (500 µL volume) containing 80 µL affinity media per column, Tecan
Pack size
96
Price
2299 USD
Part no.
PTX-91-10-01
Name
Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix
Pack size
96
Price
719 USD
Part no.
PTH-91-10-01
Name
Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton
Pack size
96
Price
719 USD
Part no.
PTR-91-10-01
Name
Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA2/ME
Pack size
96
Price
719 USD
Part no.
PTT-91-10-01
Name
Protein A PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan
Pack size
96
Price
719 USD
Part no.
PTB-91-16-01
Name
Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman
Pack size
96
Price
2463 USD
Part no.
PTX-91-16-01
Name
Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix
Pack size
96
Price
2463 USD
Part no.
PTH-91-16-01
Name
Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton
Pack size
96
Price
2463 USD
Part no.
PTR-91-16-01
Name
Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA2/ME
Pack size
96
Price
2463 USD
Part no.
PTT-91-16-01
Name
Protein A PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan
Pack size
96
Price
2463 USD
Part no.
PTB-91-20-01
Name
Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman
Pack size
96
Price
827 USD
Part no.
PTH-91-20-01
Name
Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton
Pack size
96
Price
827 USD
Part no.
PTT-91-20-01
Name
Protein A PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan
Pack size
96
Price
827 USD
Part no.
PTR-91-32-01
Name
Protein A PhyTip® columns (1 mL volume) containing 320 µL affinity media per column, MEA2/ME
Pack size
96
Price
2840 USD
Part no.
PTH-91-40-01
Name
Protein A PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton
Pack size
96
Price
1168 USD
Part no.
PTR-91-40-01
Name
Protein A PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, MEA2/ME
Pack size
96
Price
1168 USD
Part no.
PTT-91-40-01
Name
Protein A PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan
Pack size
96
Price
1168 USD
Part no.
PTB-91-80-01
Name
Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman
Pack size
96
Price
1851 USD
Part no.
PTH-91-80-01
Name
Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton
Pack size
96
Price
1851 USD
Part no.
PTT-91-80-01
Name
Protein A PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan
Pack size
96
Price
1851 USD
Part no.
PTV-92-20-01
Name
Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Agilent Bravo
Pack size
96
Price
827 USD
Part no.
PTB-92-20-01
Name
Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman
Pack size
96
Price
827 USD
Part no.
PTR-92-20-01
Name
Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA2/ME
Pack size
96
Price
827 USD
Part no.
PTT-92-20-01
Name
Protein A PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan
Pack size
96
Price
827 USD
Part no.
PTB-92-05-01
Name
Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman
Pack size
96
Price
587 USD
Part no.
PTR-92-05-01
Name
Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA2/ME
Pack size
96
Price
587 USD
Part no.
PTT-92-05-01
Name
Protein A PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan
Pack size
96
Price
587 USD
Part no.
PTX-93-20-01
Name
Protein A PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix
Pack size
96
Price
827 USD
Part no.
PTX-93-05-01
Name
Protein A PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix
Pack size
96
Price
587 USD
Part no.
PTT-95-20-01
Name
Protein A PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan
Pack size
96
Price
827 USD
Part no.
PTT-95-40-01
Name
Protein A PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan
Pack size
96
Price
1168 USD
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