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ISOLUTE® Myco

Solid Phase Extraction Columns for Mycotoxin Analysis

ISOLUTE Myco Grains and nuts
ISOLUTE Myco Column ISOLUTE Myco columns Two ISOLUTE Myco Columns Biotage sample preparation column size table ISOLUTE Myco 60 mg/3 mL (Tabless)

One product with multiple applications, simplifying and streamlining your mycotoxin analysis procedures.

CONSUMABLES

Part No. Description Product type Pack Size Price
150-0006-BG ISOLUTE Myco 60 mg/3 mL (Tabless) Consumables 50 Log in for price

ISOLUTE® Myco SPE columns offer simple and efficient multiple mycotoxin sample preparation from a wide range of matrices, ideally suited for selective and fast LC-MS/MS analysis. ISOLUTE Myco SPE columns contain a novel polymer-based sorbent designed specifically to be selective enough to isolate a wide variety of different mycotoxins. One product with multiple applications, simplifying and streamlining your mycotoxin analysis procedures.

  • Each method confidently meets the maximum residue limits (MRL) set down by EU and US regulations in terms of detection limits, %RSD and recoveries, in most cases far exceeding these requirements.
  • ISOLUTE® Myco clean up methods are quick, simple to use and require no offline steps; making the technique suitable for automation, especially useful for high throughput testing laboratories.
  • Unlike immunoaffinity columns, ISOLUTE® Myco columns are cost effective, easy to use and have no special storage requirements.

Mycotoxins Quantified Using ISOLUTE® Myco

  • aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, fumonisin B1, T-2 toxin, HT-2 toxin, zearalenone, deoxynivalenol, ergocornine, 
    a-ergocryptine, patulin

Matrices Tested Using ISOLUTE® Myco

  • grain (wheat flour, wheat, maize, barley), apple juice, nuts (Brazil nuts, peanuts)

Mycotoxins are a group of toxic metabolites produced by molds found on food crops. They can enter the food chain either through direct contamination of crops or through livestock feed and then subsequent contamination of farmed meat and dairy products. Their potential to cause harm to humans, crops and farmed animals means that a wide range of food matrices need to be tested for mycotoxin contamination. The diversity of both analyte structure and food matrix combine to produce a unique analytical challenge.

Traditionally, mycotoxin analysis has been performed with difficult to use, expensive immunoaffinity based sample preparation followed by high performance liquid chromatography (HPLC) with UV or fluorescence based detection. Due to the highly specific nature of the immunoaffinity approach, these methods allow determination of individual (or closely related) mycotoxins only, meaning multiple analyses may be required to quantify all the regulated mycotoxins in a particular sample.

Many laboratories would prefer to analyze multiple mycotoxins from each matrix with just one analytical process. The increasing adoption of liquid chromatography-tandem mass spectrometry (LC-MS/MS) based analysis facilitates this multi-analyte approach. Due to the selective nature of LC-MS/MS analysis, mycotoxins, even with similar molecular structures can be resolved at very low levels of detection in one quick assay. Sample preparation and clean up before LC-MS/MS analysis, just like in HPLC testing, is essential for robust, reproducible results, especially when dealing with complex and variable sample types. However traditional sample preparation approaches that can only isolate individual analytes/classes do not match up with this analytical approach.

Unlike more complicated and expensive approaches such as immunoaffinity assays, ISOLUTE Myco employs a simple catch and release solid phase extraction (SPE) procedure to selectively bind mycotoxins of interest to the sorbent, whilst washing off matrix components that could interfere with the analysis. Once interferences have been removed, the mycotoxins of interest can be eluted from the column and quantitatively analyzed by LC-MS/MS. The whole clean up procedure is only eight steps and is fully automation compatible.

Methods developed using ISOLUTE Myco have been designed to extract mycotoxins relevant to each sample matrix according to EU and US regulations.

Each sample type has its own challenges in terms of the interferences present, so matrix specific application notes have been developed to ensure sufficient sample clean up for robust, reliable analysis by LC-MS/MS. 

Sample Matix

Analyte Tested1

Meets MRL2

Grain
(wheat, maize, barley)
aflatoxin B1, aflatoxin B2, aflatoxin G1,
aflatoxin G2, ochratoxin A, fumonisin B1
T-2 toxin, HT-2 toxin, zearalenone
Deoxynivalenol, ergocornine
a-ergocryptine

Apple Juice Patulin

Nuts
(Brazil nuts)
aflatoxin B1, aflatoxin B2, aflatoxin G1,
aflatoxin G2, ochratoxin A, fumonisin B1
T-2 toxin, HT-2 toxin, zearalenone
Deoxynivalenol, ergocornine
a-ergocryptine

     

1Applications have been developed using representative analytes from multiple mycotoxin classes, as shown, and may be extended to include other analytes with similar chemical and structural characteristics as required.

2COMMISSION REGULATION (EC) 401/2006 (ii) (laying down the methods 
of sampling and analysis for the official control of the levels of mycotoxins
in foodstuffs).

The ISOLUTE® Myco format is compatible with most standard SPE processing equipment, including vacuum and positive pressure manifolds, and automated systems that hold the standard 3 mL column size.

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