Plasmid purification is an essential step in the transient transfection and cloning workflows. However, typical procedures are manual processes that are time consuming and laborious, and tie up highly skilled staff in routine procedures. Biotage introduce the Biotage PhyPrep, the only automated plasmid purifier capable of operating on the maxi, mega and giga plasmid preparation scales.
Biotage has developed the first automated plasmid purification system capable of performing purifications across the three scales - maxi, mega and giga prep. Using advanced robotics and novel column designs, the Biotage PhyPrep frees up biologists from laborious and time consuming manual operations.
Many researchers are highly skilled and experienced scientists, and their time is valuable. The Biotage PhyPrep frees them from laborious, time-consuming and repetitive manual operations which are vital to the transfection and biomolecule workflows, allowing skilled individuals to focus on valued added activities within the laboratory.
Following Biotage protocols delivers endotoxin-free* plasmid samples ready for further study with no further sample clean up required.
* With rich media and high copy plasmids
Pre-aliquoted reagents means sample preparation of cell pellets prior to purification is simple and effective. No need to accurately dispense buffers, instead all of the appropriate buffers are supplied in defined amounts ready for immediate use.
The PhyPrep can operate across three scales of plasmid purification - maxi, mega and giga scales. Highly pure endotoxin-free plasmid from 1mg to 10mg, automated.
A simple software interface makes programming the PhyPrep at any scale easy. Just select your scale and you are ready to go.
Preparing cell pellets for the PhyPrep involves a simple pre-aliquoted set of reagents giving reproducible results without the need for pipetting.
The Biotage PhyPrep system is designed to work with specific kits at each purification scale. Each kit contains all the components needed to ensure a high quality, endotoxin-free* purification.
*With rich media and high copy plasmids
The Biotage approach to the preparation of cell pellets involves pre-aliquoted solutions which, when combined with a cell pellet in a simple procedure, gives fast, reproducible and fool-proof sample preparation ready for purification at the specified scale. No need to accurately dispense buffers, instead all of the appropriate buffers are supplied in defined amounts ready for immediate use.
Each kit contains the required transfer tubes and PhyPrep tubes as needed for each purification. Everything required is included.
Part No. | Description |
---|---|
416700 | Biotage PhyPrep Maxi Mega Giga PhyPrep plasmid purification system. |
356330SP | Vacuum pump ME1C |
417550SP | Accessory Kit, Vacuum pump ME1C PhyPrep |
DPM-16-05-72-KIT | Biotage Plasmid MaxiPrep kit Endotoxin-Free Plasmid purification kit for 16 samples. |
DPM-16-10-72-KIT | Biotage Plasmid MegaPrep kit Endotoxin-Free Plasmid purification kit for 16 samples. |
DPM-08-20-72-KIT | Biotage Plasmid GigaPrep kit Endotoxin-Free Plasmid purification kit for 8 samples. |
417458SP | 50 x PhyPrep Tubes for Biotage®PhyPrep |
DPM-00-10-00 | 100 x 20 mL transfer tips forBiotage® PhyPrep |
DPM-00-25-00 | 25 x 20 mL transfer tips forBiotage® PhyPrep |
Poster comparing the Biotage PhyPrep Maxi Mega Giga with manual methods of plasmid purification.
Application information on plasmid purification with the Biotage® PhyPrep Maxi Mega Giga.
Quickstart guide to get up and running with PhyPrep.
Important safety information for the PhyPrep system.
Biotage offers a variety of service agreements to choose from.
Biotage is a Global Impact Tech Company committed to solving society’s problems. We offer workflow solutions and products to customers in drug discovery and development and diagnostics, analytical testing and water and environmental testing.
Biotage is contributing to sustainable science with the goal to make the world healthier, greener and cleaner – HumanKind Unlimited.
Our customers span a broad range of market segments including pharmaceutical, biotech, diagnostic, contract research and contract manufacturers as well as clinical, forensic and academic laboratories in addition to organizations focused on food safety, clean water and environmental sustainability.
Biotage is headquartered in Uppsala in Sweden and employs over 500 people worldwide. The Group had sales of 1,566 MSEK in 2022 and our products are sold in more than 80 countries. Biotage’s share (BIOT) is listed in the Large Cap segment on the NASDAQ Stockholm.
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