Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates, 1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns.
Each port of the Biotage® PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency.
Each port of the Biotage® PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency. Unlike vacuum manifolds, in which faster flowing wells and columns dry before the processing run is completed, the independent ports of the Biotage® PRESSURE+ manifolds deliver consistent output. This allows the same motive force to be applied to every cartridge and well, regardless of the contents.
Biotage® PRESSURE+ Positive Pressure manifolds allow for gas pressures of up to 100 psi, enabling greater pressure and force to be used with viscous samples. Smooth and immediate flow responses are achieved by adjusting gas pressure up or down through two regulators and a rotometer.
The self-adjusting upper manifold of the Biotage® PRESSURE+96 manifold is compatible with all 96 well plate formats in addition to the popular 1 mL and 2 mL modular array well formats without the need to purchase supplementary gaskets. Biotage collection plates are recommended for the most consistent and reliable results.
The same self-adjusting technology utilized in the Biotage PRESSURE+ 96 manifold allows Biotage® PRESSURE+ 48 to utilize all columns up to 6 mL without the need to purchase supplementary gaskets. In addition, the unique design allows for between 1 and 48 columns to be processed without empty ports affecting flow rates. Tabless or flangeless columns should be used for full population and optimum sealing. The modular rack system accommodates most popular collection vessels to be used with each cartridge size.
By using stackable collection racks and extraction racks on the Biotage® PRESSURE+ 48 manifold, many combinations are possible to accommodate varying methodologies. Racks only fit the system in one direction, ensuring consistent processing throughout.
The intuitive Biotage® PRESSURE+ is easily incorporated into laboratory work flow. Simply stack 96 well plates and collection plates (for Biotage® PRESSURE+ 96) or column rack and collection racks (for Biotage® PRESSURE+ 48) on the platform tray using the positioning markers and slide the tray under the gasket until it hits the stop. Rocker switches pressed simultaneously on the side/front of the unit lowers the gasket and compress it onto the plates/columns. This simultaneous depression of the rocker switches is a safety feature that ensures hands are kept clear of moving parts during operation.