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ISOLUTE® PE-AX
SPE Columns
ISOLUTE® PE-AX is a strong anion exchange sorbent used to extract acidic analytes from aqueous samples.
| Chemical description | Quaternary amine (non-endcapped) functionalized silica with acetate counter ion. Manufactured using a trifunctional silane. |
| Sorbent type | Strong anion exchange |
| Average particle size | 50 µm |
| Pore diameter | 60 Å |
| Exchange capacity | 0.6 meq/g |
» APPLICATION NOTES
Purification of Acidic Products from Organic Synthesis Mixtures using ISOLUTE PE-AX Columns and 96-well Plates
This Chemistry Data Sheet details the use of ISOLUTE PE-AX, a strong anion exchange sorbent, for the
purification of acidic products from organic synthesis mixtures containing non-acidic reagents or by-products.
Tags: Application Notes,
Product Notes
» MANUALS & USER GUIDES
QuickStart Guide to SPE
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
Step By Step Guide To SPE Method Development
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
» MATERIAL SAFETY DATA SHEETS
Safety Data Sheet ISOLUTE SCX-2 / PE-AX
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
Safety Data Sheet ISOLUTE P. E. – A. X.
ISOLUTE P. E. – A. X.
9503
Laboratory chemical
Tags: English,
Material Safety Data Sheets
» PRODUCT NOTES
Method Development Guidlines: Solid Phase Extraction Using ISOLUTE® SAX and ISOLUTE® PE-AX SPE Sorbents for the Extraction of Aqueous Samples
The ISOLUTE® family of anion exchange sorbents are used to extract organic anions (acid compounds capable of exhibiting a negative charge) from both aqueous and non-aqueous matrixes. This technical
note describes their use for aqueous matrices only. Although extraction using these sorbents is by the same mechanism, each has properties which influence the way they are used.
Tags: English,
Product Notes
Purification of Acidic Products from Organic Synthesis Mixtures using ISOLUTE PE-AX Columns and 96-well Plates
This Chemistry Data Sheet details the use of ISOLUTE PE-AX, a strong anion exchange sorbent, for the
purification of acidic products from organic synthesis mixtures containing non-acidic reagents or by-products.
Tags: Application Notes,
Product Notes
Search all documents »
News
13 August 2018New Findings presented at ASMS 2018
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...
11 January 2018The 2018 Analytical Catalog is Here
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17 July 2017Biotage Goes Direct in Italy
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...
28 June 2017Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...
15 June 2017Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...
20 March 2017Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
20 February 2017Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...
05 January 20162016 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
11 August 2015Why Dilute and Shoot Ruins Your Results
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...
27 March 2015New Video – Thirty Years of Packing Flash Columns
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
08 January 20152015 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
Q&A
SPE column capacity
01 July 2013Question:
What is the maximum capacity of an SPE column?
Answer:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
Browse more questions and answers >
ISOLUTE® PE-AX is a quaternary amine bonded sorbent used for the extraction of acidic compounds from aqueous (or partially aqueous) samples. It keeps a permanent positive charge across the pH range. Analytes are eluted from ISOLUTE PE-AX by eliminating the charge on the analyte (low pH), so this sorbent is not suitable for extraction of strong acids. ISOLUTE PE-AX is supplied with an acetate counter ion for more efficient extraction of acidic analytes including those with highly polar/water soluble characteristics.
ISOLUTE PE-AX is available in a wide range of SPE column formats.
ISOLUTE PE-AX is also available as loose sorbent (100g pack).
CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
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503-0002-A | ISOLUTE® PE-AX 25 mg/1 mL | Consumables | 100 | Log in for price | |
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503-0010-A | ISOLUTE® PE-AX 100 mg/1 mL | Consumables | 100 | Log in for price | |
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503-0010-B | ISOLUTE® PE-AX 100 mg/3 mL | Consumables | 50 | Log in for price | |
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503-0010-C | ISOLUTE® PE-AX 100 mg/6 mL | Consumables | 30 | Log in for price | |
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503-0020-B | ISOLUTE® PE-AX 200 mg/3 mL | Consumables | 50 | Log in for price | |
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503-0050-B | ISOLUTE® PE-AX 500 mg/3 mL | Consumables | 50 | Log in for price | |
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503-0050-C | ISOLUTE® PE-AX 500 mg/6 mL | Consumables | 30 | Log in for price | |
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503-0100-C | ISOLUTE® PE-AX 1 g/6 mL | Consumables | 30 | Log in for price | |
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503-0200-D | ISOLUTE® PE-AX 2 g/15 mL | Consumables | 20 | Log in for price | |