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ISOLUTE® SLE+ Supported Liquid Extraction Products

Load-Wait-Elute Sample Preparation

820-0400-P01
121-1010 820-0140-C 820-0140-CG 820-1420-F 820-0055-B-500 820-0200-P01 820-1000-Q01 820-0140-C isolute array base plate Biotage sample preparation column size table 820-0055-B 820-0055-B 820-0055-b-500 820-0055-BG 820-0055-BG SLE+ load-wash-elute method 820-0690-E 820-0690-E 820-0140-c-1000 820-0140-c-1000 820-0140-c 820-0140-c 820-0200-T 820-0200-T 820-0140-cg 820-0140-cg 820-0140-cg-1000 820-0140-cg-1000 820-0290-D 820-0290-D 820-0400-t 820-0400-t 820-0290-d-1000

ISOLUTE® SLE+ Supported Liquid Extraction plates and columns are designed to provide stress free extraction of analytes from biological fluids, with high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple.

 

ISOLUTE® SLE+ plates and columns are used for the supported liquid extraction of a diverse range of analytes from aqueous samples such as biological fluids (including whole blood, plasma, serum, urine and oral fluid), using a simple load-wait-elute procedure.

ISOLUTE SLE+ products are easy to use, perfectly suited to automated procedures, and column and plate formats are available for extraction of sample volumes from 10 µL to 10 mL.  See our new 48-well plate format for high throughput extraction of 1 mL sample volumes.

High analyte recoveries are obtained without any offline steps such as protein precipitation, and sample extracts are free of proteins and phospholipids. 

Features

  • Protein and phospholipid free extracts
  • High analyte recovery with no emulsion formation
  • Fast load-wait-elute procedure
  • Easy to automate for plates and columns
  • 48-well plate format now available

Supported Liquid Extraction

The Supported Liquid Extraction (SLE) process is analogous to traditional liquid-liquid extraction (LLE) and utilizes the same water immiscible solvent systems for analyte extraction.  Instead of shaking the two immiscible phases together, in SLE, the aqueous sample is immobilised on an inert support, and the organic phase flows through the support, eliminating problems such as emulsion formation, and low analyte recoveries.

Processing conditions

Processing ISOLUTE® SLE+ columns, 48-well and 96-well plates is largely performed under gravity, with a pulse of vacuum or positive pressure used to initiate loading of the sample, and to maximise solvent recovery (leading to more reproducible analyte recovery) after elution.  Both manual and automated, vacuum or pressure systems can be used.

Protein and phospholipid free extracts

Liquid-liquid extraction can give particularly clean extracts of biological fluids, as matrix components such as proteins, phospholipids and salts are not soluble in typical LLE solvents, and are excluded from the final extract.  Because the same water immiscible solvents are used in SLE, proteins and phospholipids are efficiently removed from the final extract, and no additional steps such as protein precipitation are required.

Volume  guidelines

Unlike SPE, where the sample is loaded onto the column and flows through the sorbent to waste, in SLE the ENTIRE sample volume is absorbed onto the extraction bed.   It is therefore vital to use a format with sufficient capacity to absorb the whole sample volume.  Load volume is included in each product description.

Table 1 Maximum sample and elution volumes for ISOLUTE® SLE+ products

Product capacity

Maximum load volume

Elution protocol / volume

200 µL 96-well plate

200 µL

1 x 1 mL

400 µL 96-well plate

400 µL

2 x 900 µL

1 mL 48-well plate

1 mL 5 x 1 mL

200 µL Array column/plate

200 µL 2 x 500 µL

400 µL Array column/plate

400 µL

3 x 750 µL

400 µL column

400 µL

2 x 900 µL

1 mL column

1 mL

2 x 2.5 mL

2 mL column

2 mL

2 x 5 mL

5 mL column

5 mL

3 x 8 mL

10 mL column

10 mL

2 x 20 mL

 

Comprehensive User Guide

The ISOLUTE® SLE+ User Guide describes how to develop supported liquid extraction methods using ISOLUTE SLE+ products, along with hints and tips for optimising performance and extending the range that can be extracted.

Applications

A number of application notes are available describing the use of ISOLUTE SLE+ in the extraction of therapeutic drugs, drugs of abuse and their metabolites, vitamins, steroid hormones and others.

CONSUMABLES

Part No. Description Product type Pack Size Price
121-5202 Collection Plate, 1 mL Square Consumables 50 Log in for price
121-5203 Collection Plate, 2 mL Square Consumables 50 Log in for price
121-5210 Collection Plate, 5 mL, 48-well Consumables 20 Log in for price
121-5213 Collection Plate, 2 mL Round Consumables 50 Log in for price
820-0055-B ISOLUTE® SLE+ 400 uL Sample Volume Consumables 50 Log in for price
820-0055-B-500 ISOLUTE® SLE+ 400 µL Sample Volume, Bulk Pack 500 Consumables 500 Log in for price
820-0055-BG ISOLUTE® SLE+ 400 µL Sample Volume (Tabless) Consumables 50 Log in for price
820-0055-BG-500 ISOLUTE® SLE+ 400 µL Sample Volume (Tabless), Bulk Pack 500 Consumables 500 Log in for price
820-0140-C ISOLUTE® SLE+ 1 mL Sample Volume Consumables 30 Log in for price
820-0140-C-1000 ISOLUTE® SLE+ 1 mL Sample Volume, Bulk Pack 1000 Consumables 1000 Log in for price
820-0140-CG ISOLUTE® SLE+ 1 mL Sample Volume (Tabless) Consumables 30 Log in for price
820-0140-CG-1000 ISOLUTE® SLE+ 1 mL Sample Volume (Tabless), Bulk Pack 1000 Consumables 1000 Log in for price
820-0200-P01 ISOLUTE® SLE+ 200 µL Supported Liquid Extraction plate Consumables 1 Log in for price
820-0200-T ISOLUTE® SLE+ 200 µL Array Wells (2 mL Wells) Consumables 100 Log in for price
820-0290-D ISOLUTE® SLE+ 2 mL Sample Volume Consumables 20 Log in for price
820-0290-D-1000 ISOLUTE® SLE+ 2 mL Sample Volume, Bulk Pack 1000 Consumables 1000 Log in for price
820-0400-P01 ISOLUTE® SLE+ 400 µL Supported Liquid Extraction plate Consumables 1 Log in for price
820-0400-T ISOLUTE® SLE+ 400 µL Array Wells (2 mL Wells) Consumables 100 Log in for price
820-0690-E ISOLUTE® SLE+ 5 mL Sample Volume Consumables 20 Log in for price
820-1000-Q01 ISOLUTE® SLE+ 1 mL Supported Liquid Extraction Plate (48-well) Consumables 1 Log in for price
820-1420-F ISOLUTE® SLE+ 10 mL Sample Volume Consumables 16 Log in for price