Biotage^® Isolera LS Systems

Step-gradients can be short and powerful providing separations for one or more compounds but can be challenging to develop and optimize. Isolera Spektra provides gradient optimization through new, patent-pending TLC-to-Step Gradient technology. Using solvent and TLC Rf values, Isolera Spektra builds a gradient to separate all the compounds (up to 6) in the sample. This new technology will also provide cartridge selection guidance based on the cartridge loading capacity and purification speed. The step gradient can also be used to isolate a targeted compound reducing run time and solvent use further.

PDA scanning and λ-All technology detects any UV absorbing compound eluting from a flash cartridge while measuring and displaying each eluting compound’s individual UV spectrum. Combined with novel baseline rise correction, yield losses to improper wavelength selection and large fraction volumes are no longer a concern. The PDA spectra can be reviewed and used to determine fraction purity eliminating the need for post-flash purity analysis.

Use up to four solvents in a single gradient to easily purify samples with diverse polarity. With Quatro-binary gradient capability traditional binary gradients with a limited polarity range can be adjusted to elute very lipophilic and highly polar compounds within a single purification or compound solubility during the separation by adding a constant amount of a co-solvent, acid, or base with the Isolera advanced pump.

Isolera™ methods can easily be edited either in front of the Isolera or from the comfort of your office. Edit the gradient (click & drag points AND segments), flow rate, collection volume, fraction wavelengths and modes, and add more collection racks if you need to – all while the run is in progress. Gradient changes can now be made with a simplified graphical interface or through a table layout.