High-throughput peptide purification strategies using silica-based 96-well plates

Peptides have returned to the forefront of drug discovery efforts as unbiased screening technologies have improved and the rules defining passive cell permeability have been clarified. As a result, the demand for synthetic peptide libraries has increased significantly, particularly during secondary screening as lead compound programs progress. At these early discovery phases though, highly pure peptide samples may not be necessary when evaluating these compound libraries in assays. With this in mind, and given the improvements in automated peptide library synthesis strategies, a solid phase extraction methodology may be appropriate to parallelize peptide library clean up and significantly reduce library purification time.

Literature number: P263