Sample preparation using ISOLUTE® PPT+ protein precipitation plates

Protein precipitation is a routinely used, high throughput sample preparation technique for removal of protein from biological fluid (plasma, serum or blood) samples prior to analysis by LC-MS/MS. Historically, protein precipitation has been carried out in vials or collection plates, followed by centrifugation; the supernatant is then transferred for analysis. Protein precipitation by filtration in the 96-well format has more recently been used as a high throughput, easy to automate alternative to the traditional centrifugation based technique.

Literature number: TN130.V.2