Showing posts written by Elizabeth Denton
Unbiased peptide screening technologies have evolved, improved and recently gained acceptance by the community as strategies for identifying new lead compounds against old targets with in novel chemic...
It never fails. A sequence looks relatively straightforward on paper and it's of moderate length so you start synthesizing without too much thought into the protocol. Your synthesis is finished and yo...
Our seasoned peptide chemist Dr. Elizabeth Denton has tried it all when it comes to peptides. In this short video she shows that using DMSO (dimethyl sulfoxide) in a peptide synthesis workflow is perf...
The diversity of amino acid side chain functionalities, coupled with secondary structure, gives peptides and proteins their unique properties and activities. However, when it comes to chemically synth...
What is the main goal of a peptide chemist? Elizabeth Denton, Ph.D., explains how Biotage sees the peptide synthesis workflow and how we focus on shortening the process time for scientists.
In today’s post I’ll answer the above question by comparing the crude purity of peptides synthesized using amino acid stock solutions or freshly dissolved amino acids.
Synthesizing libraries of peptides - potentially hundreds in a single synthesizer setup - has seen a resurgence of late. These synthetic libraries, often prepared on very small synthesis scales, are r...
Breakthrough during reversed phase purification often occurs for only a couple of reasons: 1) the dissolution solvent is too strong and prevents the compound from fully interacting with the stationary...
While many of the standard amino acids can be purchased pre-loaded onto Wang type resins, there are still cases where coupling the first amino acid onto Wang resin manually is necessary. In my case, a...
We’ve all done it. Loaded a very small amount of our peptide onto an analytical, maybe semi-prep column, to see where the desired peak elutes, then create a shortened version of that gradient for the ...