Documents | A High-Throughput SPE Method for Extraction of Vitamin B3 (Niacin) and Related Metabolites from Serum Using ISOLUTE® SCX-3 Prior to LC-MS/MS

A High-Throughput SPE Method for Extraction of Vitamin B3 (Niacin) and Related Metabolites from Serum Using ISOLUTE® SCX-3 Prior to LC-MS/MS

This application note describes the extraction of Vitamin B3 (Niacin, nicotinic acid) and its key metabolites from serum using a strong cation exchange SPE retention mechanism. High reproducible analyte recoveries are achieved.

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A High-Throughput SPE Method for Extraction of Vitamin B3 (Niacin) and Related Metabolites from Serum Using ISOLUTE® SCX-3 Prior to LC-MS/MS