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LC-MS/MS determination of low-molecular-weight analytes in human urine and serum after automated sample preparation

Clinical, forensic and doping testing Poster

Automated sample preparation offers significant advantages for diagnostic laboratories performing assays with high sample loads and labor-intensive manual steps. At Semmelweis University in Hungary, the measurement of cortisol in urine and symmetric dimethylarginine (SDMA) in serum is routinely performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Multi-step sample preparation is carried out with 96-well deep-well plates. For cortisol analysis, urine samples are processed using supported liquid extraction (SLE), while serum is prepared for SDMA analysis using protein precipitation (PPT).

This poster presents the feasibility of automating these workflows using the novel and versatile Biotage® Extrahera™ LV-200 sample preparation platform. 

Literature number: P286 

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