Application of a novel automated sample preparation platform for the determination of acrylamide in instant coffee

Acrylamide is a small polar, highly water-soluble molecule formed when carbohydrate rich foodstuffs are cooked at high temperatures. Due to its neurotoxic and carcinogenic properties there has been an increased demand for reliable and accurate methods for acrylamide detection. By 2017, regulatory authorities established benchmark levels and recommended that companies continue to monitor and minimize the acrylamide levels in heat processed foods. This poster demonstrates the application of EN16618:2015, involving a two-step solid phase extraction (SPE) approach for the analysis of acrylamide
in instant coffee followed by UPLC-MS/MS analysis. Subsequent method conversion to an automated sample preparation platform will be presented for assay speed, throughput, and overall workflow advantages.

Literature number: P262