A comparison of current methodologies for extraction of DoA from urine

Urine is a very useful matrix in forensic laboratories, because it is easy to collect and can help determine if a drug has been used recently. There are many viable sample preparation options for urine samples, which include phospholipid depletion, supported
liquid extraction, solid phase extraction, and more novel technologies, like dual-mode extraction. These sample preparation techniques each offer different levels of cleanliness and analyte recovery. Results from a drugs of abuse panel containing 100 compounds in urine were compared by LC-MS/MS using the different extraction techniques and popular hydrolysis procedures to identify practical considerations during method development. 

Literature number: P207