Determination of optimal sample size and microelution without dry down using SPE for a DoA panel

Drugs of abuse (DOA) testing is vital for many clinical, forensic, and pain management laboratories One hindrance with common testing methods is that patient samples are not always available in high quantities. Therefore, it is important to use as little sample as possible during the extraction process, without detrimentally effecting DOA recoveries. Many laboratories find it necessary to include as many DOA analytes as possible within a single extraction method to maximize time and cost savings during analysis. An extraction protocol using a 10 mg mixed-mode cation exchange sorbent (EVOLUTE® EXPRESS CX) was developed and various sample sizes were assessed to determine the optimal sample volume for a 98-compound DOA panel. A method involving micro elution off of the sorbent requiring no evaporation or reconstitution steps were also developed.

Literature number: P201.V.1