Evaluation of a novel low-volume 96-well SPE format for forensic and clinical toxicology

Miniaturisation within various fields of analytical chemistry is not a new phenomenon. Early phase small animal drug trials have largely been the driver due to limited sample sizes available. However, this trend is gaining popularity in forensic/clinical toxicology with respect to alleviating patient discomfort, particularly in paediatrics. Increased LC-MS/MS sensitivity has reignited this focus area. This poster will evaluate a novel low-volume 96-well solid phase extraction (SPE) format and potential application to forensic and clinical toxicology. Emphasis on matrix load volumes, wash and elution volume minimisation and processing will be investigated for: target analyte recoveries, RSDs, matrix effects and overall signal. Comparison of the novel low-volume plate and traditional SPE plate formats will be presented.

Literature number: P218