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Method optimization for the low level detection of vitamin B7 from human serum

By Biotage

Clinical, forensic and doping testing Poster

Part of the B complex group of water soluble vitamins, vitamin B7 is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize sensitivity allowing low level analysis of vitamin B7 from human serum. Method parameters: ionization mode polarity, precursor ion selection, MRM transitions, chromatography and LC mobile phase additives along with solid phase extraction protocols were optimized for increased sensitivity.  

Literature number: P154.V.1

 

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