Simplified sample preparation for DoA extraction from urine samples

Dilute and shoot (D/S) is the most common form of sample preparation for the high throughput analysis of drugs of abuse (DoA) in urine. Although superior in extract cleanliness, solid phase extraction is sometimes deemed too complex, expensive and time consuming especially when matrix hydrolysis is required. This poster aims to demonstrate a simplified sample preparation workflow utilizing on-plate urine hydrolysis, organic solvent precipitation followed by flow through filtration and matrix component scavenging. Although more complex and expensive than dilute and shoot approaches, the up-front cost is offset by superior extract cleanliness, robustness, LC column longevity ultimately leading to increased LC/MS lifespan between cleaning cycles.

Literature number: P217