Method development for peptide purification: How to create a step gradient

Synthetic peptide purification represents the most significant bottleneck of the entire peptide workflow. Reversed-phase flash chromatography is gaining traction as a strategy to reduce this bottleneck given the significant increase in loading capacity and short purification times. Presented herein are guidelines for creating a step gradient for peptide purification, ultimately enabling complete separation of a 20 amino acid peptide from a 19 amino acid peptide impurity.

Literature number: UI467