Estrone (E1) and estradiol (E2) are female steroid hormones typically monitored in peri- and postmenopausal human serum samples to assess a number of conditions. This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum using ISOLUTE® SLE+.
An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization - two approaches often used to enhance method sensitivity. The use of ammonium hydroxide and isopropanol in the HPLC mobile phase provides a unique alternative to ammonium fluoride, allowing low levels of detection of estrogens when extracted with DCM using ISOLUTE® SLE+ for sample preparation.
Two New Methods for Extraction of a Steroid Hormone Panel from Human Urine
New Method for Extraction of a Steroid Panel from Horse Hair
New Method for Analysis of Environmental Pesticides in Water
"I Was Surprised that Everything Worked So Well". A Researcher on Moving to Automated Sample Prep
Notice of Discontinuation of Biotage® SNAP Flash Columns