Documents|Extraction of Phosphatidylethanol (PEth) Species from Whole Blood Using ISOLUTE® SLE+ Prior to HPLC-MS/MS Analysis
Extraction of Phosphatidylethanol (PEth) Species from Whole Blood Using ISOLUTE® SLE+ Prior to HPLC-MS/MS Analysis
Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis.
The method is easily automated using the Biotage® Extrahera, and details of the automated procedure are included in the application note.
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