The Fast, Affordable Way to Clean up Biological Samples for LC-MS/MS
ISOLUTE® HYDRO DME+ columns and plates provide extremely efficient removal of matrix components and hydrolysis enzyme from urine and other biological fluid samples, using a simple pass through workflow.
Ideal for drugs of abuse and pain management applications, the inclusion of Biotage® HYDRO frit technology means that urine samples can be hydrolyzed in-situ in the column, eliminating the need for post hydrolysis sample transfer steps.
Dual Mode Extraction (DME) has been developed by Biotage to provide more effective removal of matrix components from urine (and other biological fluid samples) using a combination of liquid partitioning and scavenging modes. This novel extraction technology quickly and easily removes urinary components such as pigments, salts, urea, creatinine and residual hydrolysis enzyme, leaving the sample visually clear and reducing matrix effects.
Hydrolysed urine before and after ISOLUTE HYDRO DME+ clean up
ISOLUTE® HYDRO DME+ maintains analyte sensitivity (area count) without significant drop off over multiple injections, whereas D&S sensitivity drops by >50% after 400 injections. This is because of effective matrix removal with ISOLUTE HYDRO DME+. In fact, ISOLUTE HYDRO DME+ maintains signal without significant drop off for more than twice as long.
Because the HYDRO frit technology holds all of the sample above the sorbent bed during the elevated temperature incubation process, hydrolysis efficiency when using ISOLUTE® HYDRO DME+ is equivalent to off-line hydrolysis under the same conditions. Existing hydrolysis procedures can easily be transferred to ISOLUTE® HYDRO DME+.
Biotage is a Global Impact Tech Company committed to solving society’s problems. We offer workflow solutions and products to customers in Drug Discovery and Development, Analytical Testing and Water and Environmental Testing.
Biotage is contributing to sustainable science with the goal to make the world healthier, greener and cleaner – HumanKind Unlimited.
Our customers span a broad range of market segments including Pharmaceutical, Biotech, Contract Research and Contract Manufacturers as well as Clinical, Forensic and Academic laboratories in addition to organizations focused on Food safety, Clean water and Environmental sustainability.
Biotage is headquartered in Uppsala in Sweden and employs approx. 500 people worldwide. The Group had sales of SEK 1,101 million in 2019 and our products are sold in more than 70 countries. Biotage share (BIOT) is listed in the Mid Cap segment on the NASDAQ Stockholm.