The Biotage® Flash 400 system is a complete skid-mounted system designed for kilogram scale separations. Built to last and engineered with high quality materials that comply with various cGMP standards. Available in two configurations, supporting 20Kg and 40Kg cartridges, Flash 400 is the first choice of pharmaceutical and contract manufacturing companies around the world for critical purification applications.
The Biotage® Flash 400 system is a complete skid-mounted system designed for kilogram scale separations. Built to last and engineered to perform, with compliant materials for cGMP standards, Flash 400 is the first choice of pharmaceutical and contract manufacturing companies around the world for critical purification applications.
Biotage® Flash 400 systems safely operate at 100 psi enabling fast flow rates and the use of higher viscosity solvents. Our proven radial compression technology ensures near zero “wall effects” and channelling inside each column. This maintains the bed’s stability, rendering cleaner, purer fractions in less time and higher overall product yield.
Flash 400 cartridges are so-called as they are 400 mm in diameter and routinely operate at flow rates up to 6000 mL/min. These purification systems allow you to quickly complete scale-up and production runs, saving hours or even days of purification and plant time.
Robustness and safety are key factors for any scale up system. Biotage® Flash 400 needs minimal maintenance and its design has proven to be extremely reliable. With operating pressures up to 100 psi, this system supports flow rates up to 6 liters per minute, allowing operators to save days or even weeks of project time, resulting in substantial project cost savings. The Flash 400 system uses pre-packed cartridges and radial compression, and supports two interchangeable barrels for use with either 400 mm x 300 mm (Flash 400M) or 400 mm x 600 mm (Flash 400L) cartridges. One of the compression module sizes is included in the system (the module of the other size can be ordered and is interchangeable as an option). A variety of pre-packed cartridges are available depending on the purification goal and cartridges can be custom packed with the preferred chromatography media for your process. The media in each cartridge is self-contained, improving handling and eliminating exposure to contamination and impurities or potentially high toxicity APIs.
All systems come complete with an ASME “UM” stamp, CE certification, and are certified for usage in Japan, Europe and North America. An extensive engineering documentation package, certificate of performance and certificate of compliance for validation filing accompany each system. Professional Biotage service personnel train users on proper operation, and are available for project based or yearly training sessions.
Safety is a key design criteria on all Biotage flash systems. Designed for use around large quantities of solvents Flash 400 systems can operate in an explosion proof, no sparking rated area. Air driven pump and hoists, proper grounding and pressure relief devices are some of the vital components included with every system. All systems comply with NEC Class 1, Division 1 and 2, Group C and D standards.
From routine normal phase flash chromatography to more difficult separations with reversed phase silica, activated carbon, low metal/acid washed silica, Mitsubishi Diaion™ HP20SS resins, ion exchange or other custom packed, client supplied media, Flash 400 systems are capable of addressing almost every purification need.
The Flash 400 system uses pre-packed cartridges and radial compression, and supports two interchangeable barrels for use with either 400 mm x 300 mm (Flash 400M) or 400 mm x 600 mm (Flash 400L) cartridges. One of the compression module sizes is included in the system (the module of the other size can be ordered and is interchangeable as an option). A variety of pre-packed cartridges are available depending on the purification goal and cartridges can be custom packed with the preferred chromatography media for your process. The media in each cartridge is self-contained, improving handling and eliminating exposure to contamination and impurities or potentially high toxicity APIs. Flash 400 systems are capable of addressing almost every purification need.
Technologies such as crystallization and adsorption can be difficult and time consuming to scale up. Using the extensive cartridge range from Biotage, reliable results are verifiable and easy to achieve with any separation. For example a Biotage® SNAP or SFAR 10 g column (to purify 100 mg quantities) can be used as a basis to purify multi gram and ultimately kg quantities of product using a 40 kg Flash 400 cartridge. Moving up the cartridge range, purifications can be scaled up 4000 times. We recommend increments of 10-fold to provide maximum control and opportunity to optimize further any scale up parameters.
Part No. | Description | Pack Size | List Price | |
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SF-521-50150 | Flash 400L system (with Hoist) | 1 | Add to quote/cart | |
SF-521-50070 | Flash 400M system (with Hoist) | 1 | Add to quote/cart |
Part No. | Description | Pack Size | List Price | |
---|---|---|---|---|
FSUL-0401-50150 | Cartridge, (1) HP-Sphere C18 FLASH 400L | 1 | Add to quote/cart | |
FSUL-0401-50070 | Cartridge, (1) HP-Sphere C18 FLASH 400M | 1 | Add to quote/cart | |
C1YR-4021-50155 | Flash 400L Cartridge (26Kg) with WAC | 2 | Add to quote/cart | |
F400L-0445-50150 | Flash 400L Cartridge with Biotage Sfär 60 micron silica,1/pk | 1 | Add to quote/cart | |
F400L-0443-50150 | Flash 400L Cartridge with Biotage Sfär HC silica, 1pk | 1 | Add to quote/cart | |
FT6-2530-50150 | Flash 400L Cartridge with HP-20SS, 1pk | 1 | Add to quote/cart | |
F400L-0442-50150 | Flash 400L Cartridge with HP-Sphere | 1 | Add to quote/cart | |
FPNH-0454-50150 | Flash 400L Cartridge with ISOLUTE Flash-NH2, 1/pk | 1 | Add to quote/cart | |
FL0-1118-50150 | Flash 400L Cartridge with KP-C18-HS | 1 | Add to quote/cart | |
FK0-1107-50150 | Flash 400L Cartridge with KP-Sil | 1 | Add to quote/cart | |
FPNH-400L | Flash 400L with Biotage KP-NH media, 1/pk | 1 | Add to quote/cart | |
C1YR-4021-50075 | Flash 400M Cartridge (13Kg) with WAC | 2 | Add to quote/cart | |
F400M-0445-50070 | Flash 400M Cartridge with Biotage Sfär 60 micron silica,1/pk | 1 | Add to quote/cart | |
F400M-0443-50070 | Flash 400M Cartridge with Biotage Sfär HC silica, 1pk | 1 | Add to quote/cart | |
F400M-0442-50070 | Flash 400M Cartridge with HP-Sphere | 1 | Add to quote/cart | |
FPNH-0454-50070 | Flash 400M Cartridge with ISOLUTE Flash-NH2, 1/pk | 1 | Add to quote/cart | |
FL0-1118-50070 | Flash 400M Cartridge with KP-C18-HS | 1 | Add to quote/cart | |
FK0-1107-50070 | Flash 400M Cartridge with KP-Sil | 1 | Add to quote/cart | |
FPNH-400M | Flash 400M with Biotage KP-NH media, 1/pk | 1 | Add to quote/cart |
This document illustrates O-rings available for use in Biotage Scale-Up Flash System compression modules.
Essential items to help prepare for your system installation / also read in connection with engineering document package (available on request)
This paper describes a case study at CordenPharma Switzerland where the Biotage® Flash 400 large-scale chromatography system and pre-packed cartridges were evaluated alongside a custom-made alternative as part of a plan to increase the efficiency of the workflow.
This paper describes a project at Croda relating to supply of lipids for the global response to the emerging health crisis related to the spread of COVID-19.
Purify up to 8 kg of material with Biotage® Flash 400 large scale purification system. Robust and low-maintenance, powered by compressed air and nitrogen, compliant with regulatory requirements.
A short sequence showing how Biotage Flash 400 works
Part No. | Description | |
---|---|---|
SER-400-IN | Installation and Familiarization Installation and familiarization package | Add to quote |
SER-400-IQOQ | Qualification Add-on a compliance package (IQOQ) | Add to quote |
Biotage offers a variety of service agreements to choose from.
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