ISOLUTE® SLE+ Supported Liquid Extraction plates and columns are designed to provide stress free extraction of analytes from biological fluids, with high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple.
Part No. | Description | Pack Size | |
---|---|---|---|
820-0140-C | ISOLUTE® SLE+ 1 mL Sample Volume | 30 | Add to quote |
820-0140-C-1000 | ISOLUTE® SLE+ 1 mL Sample Volume | 1000 | Add to quote |
820-0140-CG | ISOLUTE® SLE+ 1 mL Sample Volume (Tabless) | 30 | Add to quote |
820-0140-CG-1000 | ISOLUTE® SLE+ 1 mL Sample Volume (Tabless) | 1000 | Add to quote |
820-1000-Q01 | ISOLUTE® SLE+ 1 mL Supported Liquid Extraction Plate (48-Well) | 1 | Add to quote |
820-1420-F | ISOLUTE® SLE+ 10 mL Sample Volume | 16 | Add to quote |
820-0290-D | ISOLUTE® SLE+ 2 mL Sample Volume | 20 | Add to quote |
820-0290-D-1000 | ISOLUTE® SLE+ 2 mL Sample Volume | 1000 | Add to quote |
820-0200-T | ISOLUTE® SLE+ 200 µL Array Wells (2 mL Wells) | 100 | Add to quote |
820-0200-P01 | ISOLUTE® SLE+ 200 µL Supported Liquid Extraction Plate | 1 | Add to quote |
820-0055-B | ISOLUTE® SLE+ 400 uL Sample Volume | 50 | Add to quote |
820-0400-T | ISOLUTE® SLE+ 400 µL Array Wells (2 mL Wells) | 100 | Add to quote |
820-0400-T-1000 | ISOLUTE® SLE+ 400 µL Array Wells (2 mL Wells) (1000/pk) | 1000 | Add to quote |
820-0055-B-500 | ISOLUTE® SLE+ 400 µL Sample Volume | 500 | Add to quote |
820-0055-BG | ISOLUTE® SLE+ 400 µL Sample Volume (Tabless) | 50 | Add to quote |
820-0055-BG-500 | ISOLUTE® SLE+ 400 µL Sample Volume (Tabless) | 500 | Add to quote |
820-0400-P01 | ISOLUTE® SLE+ 400 µL Supported Liquid Extraction Plate | 1 | Add to quote |
820-0690-E | ISOLUTE® SLE+ 5 mL Sample Volume | 20 | Add to quote |
ISOLUTE® SLE+ plates and columns are used for the supported liquid extraction of a diverse range of analytes from aqueous samples such as biological fluids (including whole blood, plasma, serum, urine and oral fluid), using a simple load-wait-elute procedure.
ISOLUTE SLE+ products are easy to use, perfectly suited to automated procedures, and column and plate formats are available for extraction of sample volumes from 10 µL to 10 mL.
High analyte recoveries are obtained without any offline steps such as protein precipitation, and sample extracts are free of proteins and phospholipids.
The supported liquid extraction (SLE) process is analogous to traditional liquid-liquid extraction (LLE) and utilizes the same water immiscible solvent systems for analyte extraction. Instead of shaking the two immiscible phases together, in SLE, the aqueous sample is immobilised on an inert support, and the organic phase flows through the support, eliminating problems such as emulsion formation, and low analyte recoveries.
Liquid-liquid extraction can give particularly clean extracts of biological fluids, as matrix components such as proteins, phospholipids and salts are not soluble in typical LLE solvents, and are excluded from the final extract. Because the same water immiscible solvents are used in SLE, proteins and phospholipids are efficiently removed from the final extract, and no additional steps such as protein precipitation are required.
Unlike SPE, where the sample is loaded onto the column and flows through the sorbent to waste, in SLE the ENTIRE sample volume is absorbed onto the extraction bed. It is therefore vital to use a format with sufficient capacity to absorb the whole sample volume. Load volume is included in each product description.
Product capacity |
Maximum load volume |
Elution protocol / volume |
200 µL 96-well plate |
200 µL |
1 x 1 mL |
400 µL 96-well plate |
400 µL |
2 x 900 µL |
1 mL 48-well plate |
1 mL | 5 x 1 mL |
200 µL Array column/plate |
200 µL | 2 x 500 µL |
400 µL Array column/plate |
400 µL |
3 x 750 µL |
400 µL column |
400 µL |
2 x 900 µL |
1 mL column |
1 mL |
2 x 2.5 mL |
2 mL column |
2 mL |
2 x 5 mL |
5 mL column |
5 mL |
3 x 8 mL |
10 mL column |
10 mL |
2 x 20 mL |
The ISOLUTE® SLE+ User Guide describes how to develop supported liquid extraction methods using ISOLUTE SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted.
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