Flash purification of peptides: Exploring green solvents and strategies for peptide workflow

Traditionally, peptide purification has relied on preparative high performance liquid chromatography (prep-HPLC), known for its excellent resolution and impurity removal. However, this method is often hampered by low column loading capacity and extended purification times making it inefficient and difficult to scale. In response to these challenges, Biotage has developed strategies for sustainable flash chromatography as an alternative to prep-HPLC for the purification of synthetic peptides. Automated reversed-phase flash purification offers a fast, flexible, and cost-effective alternative to traditional methods, enabling researchers to purify synthetic peptides efficiently and redirect their efforts toward more important tasks. This approach addresses issues such as molecule-specific optimization, scale-up difficulties, and the inefficiencies of current processes, providing a greener and more efficient solution.

Literature number: P283