Sample matrix clean-up using protein and phospholipid depletion technique for DoA analysis in whole blood

Clinical analysis encompasses a wide range of analytes, whether they are endogenous compounds such as amino acids, vitamins, and hormones or exogenous compounds ("xenobiotics"), such as prescribed and/or illicit drugs and their metabolites. The diversity of these analytes' properties coupled with the complexity of sample matrices presents challenges in LC-MS/MS analysis, as it is difficult to develop a method that can remove biomatrix interferences effectively while maintaining good recovery across wide ranges of analytes. Sample extractions methods using solid phase extraction (SPE) are tailored to specific compound classes, and the extraction procedures are rather tedious and time-consuming. This underlines the need for a simple, fast, cost-effective,  and automatable sample preparation approach for clinical analysis.

Literature number: P280