ISOLUTE® HYDRO DME+ Dual Mode Extraction Products

This application note describes the extraction of 18 growth promoters (steroids, resorcylic acid lactones and stilbenes) using ISOLUTE® HYDRO DME+ prior to LC/MS analysis. LOQs are typically < 0.4 ng/mL, with a load of 75 μL of urine.
Tags: 16-ß-hydroxystanozolol, Application Notes, Column, Dienestrol, Diethylstilbestrol, English, Food, Growth Promoters, Hexestrol, Hormone, Hormones, LC-MS/MS, Methylboldenone, Methyltestosterone, Steroid, Steroid Hormones, Steroids, Taleranol, Urine (Cattle), Urine (Pig), Urine (Sheep), Urine (horse), Veterinary drugs, Zearalenone, Zeranol, ß-nortestosterone, ß-zearalenol, α-boldenone, α-nortestosterone, α-trenbolone, α-zearalenol, β-boldenone, β-trenbolone

This application note describes the extraction of 25 illicit and prescribed drugs from hydrolyzed urine using ISOLUTE® HYDRO DME+ Dual Mode Extraction plates prior to UPLC-MS/MS analysis. Because of the enhanced sample clean up delivered by ISOLUTE HYDRO DME+ products, analyte sensitivity is significantly increased compared to traditional dilute and shoot (D&S) techniques, resulting in reduced limits of quantitation (LOQ)
Tags: 6-MAM, 7-Aminoclonazepam, 7-amino-flunitrazepam, 96-well plate, Amphetamine, Amphetamines, Application Notes, Benzodiazepines, Benzoylecgonine, Buprenorphine, Clinical, Cocaine, Cocaines, Codeine, EDDP, Ecgonine Methyl Ester EME, Ecgonine methyl ester, English, Flunitrazepam, Forensic, Gabapentin, Hydrocodone, Hydrolysed urine, Ketamine, LC-MS/MS, MDMA, Mephedrone, Methadone, Morphine, Norbuprenorphine, Opioid, Oxazepam, Oxymorphone, PCP, Phencyclidine (PCP), Pregabalin, Ritalinic acid, Urine, Zaleplon

Dilute and shoot (D/S) is the most common form of sample preparation for the high throughput analysis of drugs of abuse (DoA)in urine. Although superior in extract cleanliness, solid phase extraction is sometimes deemed too complex, expensive and time consuming especially when matrix hydrolysis is required. This poster aims to demonstrate a simplified sample preparation workflow utilizing on-plate urine hydrolysis, organic solvent precipitation followed by flow through filtration and matrix component scavenging. ASMS, 2020.
Tags: 6-MAM, Amphetamine, Codeine, Drugs of Abuse, Ecgonine Methyl Ester EME, Forensic, Gabapentin, Hydrocodone, Ketamine, LC-MS/MS, MDMA, Mephedrone, Morphine, Oxymorphone, Posters, Pregabalin, Urine

This poster evaluates two “pass through” solid phase extraction techniques: (protein removal/phospholipid depletion (ISOLUTE PLD+) and dual mode extraction (ISOLUTE HYDRO DME+)) and supported liquid extraction (ISOLUTE SLE+) for a group of about 100 drugs of abuse compounds in whole blood. SOFT 2019
Tags: 96-well plate, Anticonvulsant, Antipsychotics, Barbiturates, Benzodiazepines, Cannabinoid, Clinical, English, Forensic, Hallucinogen, LC-MS/MS, Opiates, Opioid, Posters, Stimulants, Whole blood

This poster compares various sample preparation techniques (mixed-mode SPE (EVOLUTE EXPRESS CX, phospholipid depletion (ISOLUTE PLD+), supported liquid extraction (ISOLUTE SLE+) and dual mode extraction (ISOLUTE HYDRO DME+) for extraction of a panel of 98 drugs of abuse from urine. SOFT 2019
Tags: 96-well plate, Amphetamines, Anticonvulsant, Antipsychotics, Barbiturates, Benzodiazepines, Cannabinoid, Clinical, English, Fentanyls, Forensic, Hallucinogen, LC-MS/MS, Opiates, Opioid, Posters, Stimulants, z-drugs

Sample preparation is generally regarded as a pain point for many labs as it can be laborious, time consuming and relatively expensive. In a high throughput setting performing offline chemistry also leads to increases in sample turnaround time and reporting. This poster evaluates simplified sample preparation workflows using optimized sample hold-up technology allowing chemistry in-situ. Reduced labour, extraction complexity, processing time, solvent usage and resultant instrument contamination and downtime can offset upfront costs associated with sample preparation. ASMS 2019
Tags: 96-well plate, Clinical, Drugs of Abuse, English, Forensic, Hydrolysed urine, LC-MS/MS, Over the counter pharmaceuticals, Pharmaceuticals, Plasma, Posters, Urine

This poster evaluates the extraction of a range of drugs of abuse from hydrolyzed and nonhydrolyzed urine using a novel flow through scavenging product, ISOLUTE® HYDRO DME+. Matrix component removal in terms of creatinine and urea, salt residue, pigmentation associated with urobillin content and protein removal will be demonstrated. SOFT 2018
Tags: 96-well plate, Barbiturates, Benzodiazepines, Clinical, Cocaines, Column, Drugs of Abuse, English, Forensic, LC-MS/MS, Opiates, Opioid, Posters

Product Note for ISOLUTE® HYDRO DME+ Dual Mode Extraction 96-well Plates and Columns
Tags: 96-well plate, Amphetamines, Barbiturates, Benzodiazepines, Cannabinoid, Clinical, Cocaines, Column, Drugs of Abuse, English, Forensic, Hydrolysed urine, LC-MS/MS, Opiates, Opioid, Pain Management, Product Notes, Urine