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ISOLUTE® HYDRO DME+ Dual Mode Extraction Products
The Fast, Affordable Way to Clean up Biological Samples for LC-MS/MS
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Highlighted Documents
- ISOLUTE® HYDRO DME+ Dual Mode Extraction 96-well Plates and Columns
- Extraction of Illicit and Prescribed Drugs from Enzyme-Hydrolyzed Urine Using ISOLUTE® HYDRO DME+ Prior to UPLC-MS/MS Analysis
- A Comparison of Current Methodologies for Extraction of Drugs of Abuse from Urine
- Extraction of Veterinary Growth Promoters from Animal Urine Using ISOLUTE® HYDRO DME+ Prior to UPLC-MS/MS Analysis
More ISOLUTE® HYDRO DME+ Dual Mode Extraction Products documents »
ISOLUTE® HYDRO DME+ columns and plates provide extremely efficient removal of matrix components and hydrolysis enzyme from urine and other biological fluid samples, using a simple pass through workflow.
» APPLICATION NOTES
Extraction of Veterinary Growth Promoters from Animal Urine Using ISOLUTE® HYDRO DME+ Prior to UPLC-MS/MS Analysis
This application note describes the extraction of 18 growth promoters (steroids, resorcylic acid lactones and stilbenes) using ISOLUTE® HYDRO DME+ prior to LC/MS analysis. LOQs are typically < 0.4 ng/mL, with a load of 75 μL of urine.
Tags: 16-ß-hydroxystanozolol,
Application Notes,
Column,
Dienestrol,
Diethylstilbestrol,
English,
Food,
Growth Promoters,
Hexestrol,
Hormone,
Hormones,
LC-MS/MS,
Methylboldenone,
Methyltestosterone,
Steroid,
Steroid Hormones,
Steroids,
Taleranol,
Urine (Cattle),
Urine (Pig),
Urine (Sheep),
Urine (horse),
Veterinary drugs,
Zearalenone,
Zeranol,
ß-nortestosterone,
ß-zearalenol,
α-boldenone,
α-nortestosterone,
α-trenbolone,
α-zearalenol,
β-boldenone,
β-trenbolone
Extraction of Illicit and Prescribed Drugs from Enzyme-Hydrolyzed Urine Using ISOLUTE® HYDRO DME+ Prior to UPLC-MS/MS Analysis
This application note describes the extraction of 25 illicit and prescribed drugs from hydrolyzed urine using ISOLUTE® HYDRO DME+ Dual Mode Extraction plates prior to UPLC-MS/MS analysis.
Because of the enhanced sample clean up delivered by ISOLUTE HYDRO DME+ products, analyte sensitivity is significantly increased compared to traditional dilute and shoot (D&S) techniques, resulting in reduced limits of quantitation (LOQ)
Tags: 6-MAM,
7-Aminoclonazepam,
7-amino-flunitrazepam,
96-well plate,
Amphetamine,
Amphetamines,
Application Notes,
Benzodiazepines,
Benzoylecgonine,
Buprenorphine,
Clinical,
Cocaine,
Cocaines,
Codeine,
EDDP,
Ecgonine Methyl Ester EME,
Ecgonine methyl ester,
English,
Flunitrazepam,
Forensic,
Gabapentin,
Hydrocodone,
Hydrolysed urine,
Ketamine,
LC-MS/MS,
MDMA,
Mephedrone,
Methadone,
Morphine,
Norbuprenorphine,
Opioid,
Oxazepam,
Oxymorphone,
PCP,
Phencyclidine (PCP),
Pregabalin,
Ritalinic acid,
Urine,
Zaleplon
» POSTERS
Strategies for Extraction of Drugs from Whole Blood
This poster evaluates two “pass through” solid phase extraction techniques: (protein removal/phospholipid depletion (ISOLUTE PLD+) and dual mode extraction (ISOLUTE HYDRO DME+)) and supported liquid extraction (ISOLUTE SLE+) for a group of about 100 drugs of abuse compounds in whole blood.
SOFT 2019
Tags: 96-well plate,
Anticonvulsant,
Antipsychotics,
Barbiturates,
Benzodiazepines,
Cannabinoid,
Clinical,
English,
Forensic,
Hallucinogen,
LC-MS/MS,
Opiates,
Opioid,
Posters,
Stimulants,
Whole blood
A Comparison of Current Methodologies for Extraction of Drugs of Abuse from Urine
This poster compares various sample preparation techniques (mixed-mode SPE (EVOLUTE EXPRESS CX, phospholipid depletion (ISOLUTE PLD+), supported liquid extraction (ISOLUTE SLE+) and dual mode extraction (ISOLUTE HYDRO DME+) for extraction of a panel of 98 drugs of abuse from urine.
SOFT 2019
Tags: 96-well plate,
Amphetamines,
Anticonvulsant,
Antipsychotics,
Barbiturates,
Benzodiazepines,
Cannabinoid,
Clinical,
English,
Fentanyls,
Forensic,
Hallucinogen,
LC-MS/MS,
Opiates,
Opioid,
Posters,
Stimulants,
z-drugs
Evolution of Sample Preparation: Workflow Simplification Utilizing Sample Hold-up Technology in Forensic and Clinical Analyses
Sample preparation is generally regarded as a pain point for many labs as it can be laborious, time consuming and relatively expensive. In a high throughput setting performing offline chemistry also leads to increases in sample turnaround time and reporting.
This poster evaluates simplified sample preparation workflows using optimized sample hold-up technology allowing chemistry in-situ. Reduced labour, extraction complexity, processing time, solvent usage and resultant instrument contamination and downtime can offset upfront costs associated with sample preparation.
ASMS 2019
Tags: 96-well plate,
Clinical,
Drugs of Abuse,
English,
Forensic,
Hydrolysed urine,
LC-MS/MS,
Over the counter pharmaceuticals,
Pharmaceuticals,
Plasma,
Posters,
Urine
Evaluation of Matrix Component Removal Using Novel Flow-through Scavenging Plates or Columns for Drugs of Abuse Testing in Urine
This poster evaluates the extraction of a range of drugs of abuse from hydrolyzed and nonhydrolyzed urine using a novel flow through scavenging product, ISOLUTE® HYDRO DME+. Matrix component removal in terms of creatinine and urea, salt residue, pigmentation associated with urobillin content and protein removal will be demonstrated.
SOFT 2018
Tags: 96-well plate,
Barbiturates,
Benzodiazepines,
Clinical,
Cocaines,
Column,
Drugs of Abuse,
English,
Forensic,
LC-MS/MS,
Opiates,
Opioid,
Posters
» PRODUCT NOTES
ISOLUTE® HYDRO DME+ Dual Mode Extraction 96-well Plates and Columns
Product Note for ISOLUTE® HYDRO DME+ Dual Mode Extraction 96-well Plates and Columns
Tags: 96-well plate,
Amphetamines,
Barbiturates,
Benzodiazepines,
Cannabinoid,
Clinical,
Cocaines,
Column,
Drugs of Abuse,
English,
Forensic,
Hydrolysed urine,
LC-MS/MS,
Opiates,
Opioid,
Pain Management,
Product Notes,
Urine
Search all documents »
PRODUCT VIDEOS
ISOLUTE HYDRO DME+ Intro video
News
20 November 2019Two Alternative Approaches to the Extraction of a Suite of Regulated Veterinary Growth Promoters
These two application notes describe alternative approaches to the extraction of a suite of regulated veterinary growth promoters (including steroids, stilbenes and resorcylic acid lactones) from a...
24 April 2019Hydrolyze Urine Samples In-Situ - New Application Note
This application note demonstrates the applicability of our newest sample preparation consumable, ISOLUTE® HYDRO DME+ for in-situ hydrolysis and clean-up of urine samples, for the analysis of a...
13 February 2018Finally - an Easy Urine Sample Preparation for LC/MS Analysis
ISOLUTE® HYDRO DME+ is a new product targeted at drugs of abuse and pain management panels in urine applications. This novel patent pending product quickly and easily removes matrix components ...
Ideal for drugs of abuse and pain management applications, the inclusion of Biotage® HYDRO frit technology means that urine samples can be hydrolyzed in-situ in the column, eliminating the need for post hydrolysis sample transfer steps.
Figure 1. ISOLUTE® HYDRO DME+ urine clean-up workflow with in-situ hydrolysis.
Effective Sample Clean up
Dual Mode Extraction (DME) has been developed by Biotage to provide more effective removal of matrix components from urine (and other biological fluid samples) using a combination of liquid partitioning and scavenging modes. This novel extraction technology quickly and easily removes urinary components such as pigments, salts, urea, creatinine and residual hydrolysis enzyme, leaving the sample visually clear and reducing matrix effects.
Figure 2. Hydrolysed urine before and after ISOLUTE HYDRO DME+ clean up
Figure 3. ISOLUTE® HYDRO DME+ maintains analyte sensitivity (area count) without significant drop off over multiple injections, whereas D&S sensitivity drops by >50% after 400 injections. This is because of effective matrix removal with ISOLUTE HYDRO DME+. In fact, ISOLUTE HYDRO DME+ maintains signal without significant drop off for more than twice as long.
Improved analyte sensitivity
Because of the enhanced sample clean up delived by ISOLUTE HYDRO DME+ products, analyte sensitivity is significantly increased compared to dilute and shoot (D&S), resulting in reduced LOQs.
Figure 4. For the majority of analytes, sensitivity is increased by a factor of 2 to 5 or more
No Compromise on Hydrolysis Efficiency
Because the HYDRO frit technology holds all of the sample above the sorbent bed during the elevated temperature incubation process, hydrolysis efficiency when using ISOLUTE® HYDRO DME+ is equivalent to off-line hydrolysis under the same conditions. Existing hydrolysis procedures can easily be transferred to ISOLUTE® HYDRO DME+.
CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
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970-0040-BZ | ISOLUTE® HYDRO DME+ 400 mg/3 mL Columns | Consumables | 50 | Log in for price | |
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970-0400-PZ01 | ISOLUTE® HYDRO DME+ 400 mg Plate | Consumables | 1 | Log in for price | |