Biotage® Extrahera™ HV-5000
Automated sample preparation workstation
Automates high-volume SPE and SLE sample prep with SMART pipetting, GLP compliance, and intuitive software.
Explore the main benefits of Biotage® Extrahera™ HV-5000
Designed for high volume sample preparation
Biotage® Extrahera™ HV-5000 is built for labs handling large sample volumes, supporting 3–15 mL SPE and SLE cartridges. It utilizes a 4-channel pipette head capable of aspirating and dispensing 250–5000 µL per channel, enabling parallel processing of up to 48 samples with precision and speed.
SMART pipetting and workflow automation
Prevent air aspiration and overflow with SMART loading. The system adjusts pipette descent rate to match aspiration speed, ensuring clean, accurate transfers every time.
Versatile applications across industries
Validated for forensic toxicology, doping control, food safety, and environmental testing. Preloaded methods and compatible consumables ensure reliable results across diverse matrices.
Compliance and operational confidence
GLP-ready software includes audit trail and user management. The intuitive interface simplifies onboarding and daily operation, making it ideal for regulated and high-throughput labs.
Specifications
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Solvent capacity
5 auto + 5 manual reservoirs -
Dimensions
860 × 570 × 790 mm (33.8” x 22.4” x 31.1”) with touchscreen -
Weight
80 kg (176 lbs.) -
Interfaces
Touchscreen, USB 2.0, Ethernet
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Pipette pump capacity
250-5000 μL -
Pipetting specifications
For the 5000 μL pipette tip (P/N 417007):
At 250 μL: ± 2.0% accuracy and 3.0% CV
At 500 μL: ±1.5% accuracy and 2.0% CV
At 1000 μL: ±1.0% accuracy and 1.5% CV
At 5000 μL: ±0.5% accuracy and 1.0% CV -
Pressure unit
Positive pressure unit:
Processing: 0-5 Bar (0.1 Bar Increments)
Plate dry: 5 Bar at 600 mL/min -
Format
48-position extraction racks for 3 mL (tabless) columns
24-position extraction racks for 3 and 6 mL (tabless) columns
12-position extraction racks for 10 mL (tabless) columns
12-position extraction racks for 15 mL (tabless) columns
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Plate positions
6 position upper deck used for sample, extraction plates, pipette tips and solvents.
4 positions on lower deck carousel for multiple elution and waste.
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Disposable tips
Disposable sample tips (Position 2): 5000 μL clear tips
Disposable solvent tips (Position 1): 5000 μL clear tips
Disposable solvent tips (Position 6): 5000 μL clear tips
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Temperature
Operating temperature: 18°C to 32°C.
Storage and transportation temperature: -25 °C to 60 °C
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Humidity
10 to 90% RH -
Power supply
100 to 240 V, 50/60 Hz -
Fuses
T4A at the power inlet (2 required) -
Maximum power consumed
300 VA -
Pressurized air supply
>5 L/min (0.18 cubic feet/min), 6 ± 0.2 bar (0.6 ± 0.02 MPa; 87 ± 3 psi) -
Inert gas supply
AIR port requirements: >5 L/min (0.18 cubic feet/min), 6 ± 0.2 bar (0.6 ± 0.02 MPa; 87 ± 3 psi).
N2 port requirements: 1 L/min (0.04 cubic feet/min) during processing and 70 L/min (2.5 cubic feet/min) during plate dry, 6 ± 0.2 bar (0.6 ± 0.02 MPa, 87 ± 3 psi)
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Vacuum supply
Partial pressure range: < 500 mbar (50 kPa; 7 psi)
Flow rate: ≥ 8.3 L/min (0.29 cubic feet/min)
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Max sound level
65 dB(A) -
GLP
GLP software ready -
Certifications
CE, CSA certified
Resources
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Read article
Application guide
Guide to environmental applications using Biotage® Extrahera™ HV-5000
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Read article
Application note
Automated extraction of 11-Nor-9-Carboxy-THC from human urine with ISOLUTE® HAX SPE columns using Biotage® Extrahera™ HV-5000, prior to GC-MS analysis
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Read article
Application note
Automated extraction of Tetrahydrocannabinol (THC) and its metabolites from human whole blood with ISOLUTE® HAX SPE columns using Biotage® Extrahera™ HV-5000, prior to GC-MS analysis
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Read article
Application note
Automated extraction of Amphetamines from human urine using ISOLUTE® HCX SPE columns with Biotage® Extrahera™ HV-5000, prior to GC-MS analysis
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Read article
Application note
Automated extraction of a drugs of abuse panel from urine using ISOLUTE® HCX SPE columns with the Biotage® Extrahera™ HV-5000
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Read article
Application note
Automated extraction of acrylamide from instant coffee using Biotage® Extrahera™ HV-5000, according to EN 16618:2015
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Read article
Application note
Extraction of veterinary growth promoters from animal urine using ISOLUTE® SLE+ prior to UPLC-MS/MS analysis
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Read article
Application note
Extraction of a comprehensive steroid panel from horse hair using ISOLUTE® SLE+ prior to LC/MS-MS analysis
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Read article
Application note
Automated extraction of a drugs of abuse panel from human urine using ISOLUTE® SLE+ with Biotage® Extrahera™ HV-5000
Ordering information
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