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This is an interesting question that I am asked from time to time. There does seem to be two camps in which chemists reside – one believing longer and thinner columns provide better separations and th...
The bane of organic synthesis for most chemists is purification rather than synthesis. Synthetic reaction mixtures are rarely devoid of impurities so some type of purification is necessary. Most often...
Media particle size and solvent flow rate play major roles in chromatographic separations including flash purification. This is true in both reversed-phase chromatography (aka partition chromatography...
Many chemists I talk to understand that TLC data is useful for flash chromatography method development. Most also know that they should try to get their target compound to elute with an Rf between 0.1...
Silica is the most commonly used sorbent for flash column chromatography. When solvent is pumped through a column packed with dry silica you may notice it gets warm and sometimes down right hot!
Selectivity and solvent strength are the most important factors that determine success or failure of a chromatographic separation. These two independent dynamics apply to both normal- and reversed-pha...
For chemists, flash chromatography is part of their everyday synthesis workflow. For most syntheses, crude reaction mixtures are purified by normal-phase (aka adsorption) chromatography. There are tim...
Scaling up reversed-phase flash chromatography methods is often necessary as reaction scale increases. This is especially true when other non-chromatographic purification techniques do not work or mee...
As the popularity of prep-scale, reversed-phase flash chromatography increases, so does the frequency that I get asked this question, "How do I determine loading capacity in reversed-phase flash chrom...
How to choose between normal- and reversed-phase flash column chromatography is an excellent question and one that my readers often ask. Those who use column chromatography know that as long as the re...