Showing posts written by Bob Bickler
Evaporative light-scattering detection (ELSD) is an effective tool for detecting compounds with minimal to no UV absorbance. ELS detectors convert the column effluent into an aerosol by adding heated ...
I like working with natural products, at least from a chromatography perspective. The sheer breadth of compounds extractable from a plant makes separations a challenge but overcoming that challenge wi...
ELSD, or evaporative light scattering detector, is an accessory item available for many commercially available flash chromatography systems. It is typically used to detect compounds that are UV transp...
Dry loading crude reaction mixtures can dramatically improve purification results. This is a topic I have blogged about several times and is important enough to discuss again.
Are you currently or have you ever had an issue where your chromatographic trace shows a sine-wave pattern while a compound is eluting, Figure 1? If so, there are several causes, and solutions, for th...
The answer to this question is absolutely yes. The choice of organic solvent can impact your reversed phase flash purification results just as organic solvent selection impacts normal phase results.
If you use automated flash chromatography you may have a system than can have four solvents attached to it. In fact, most flash chromatography systems are designed to have as many as four solvents ava...
We all know the adage “Time is Money”. It is particularly true in pharma where the timeline to get a pharmaceutical product to market can take 10+ years and cost billions of dollars. With this as the ...
With the increasing use of reversed phase flash chromatography in medicinal chemistry and organic synthesis labs comes the desire to speed up and make these purifications more time and solvent efficie...
Have you ever had a purified reaction product, which should be white, retain color after purification? If so, what did you do to remove it?