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Reversed-phase flash chromatography is extremely useful when purifying all types of compounds. Compounds that are charged or ionizable, however, typically need either a pH modifier (acid or base) or a...
Most chemists I meet utilizing flash chromatography use linear gradients. These gradients are either very generic (X% to Y% over a certain time or volume) or can be complex with multiple slope changes...
Flash chromatography gradients need to provide as good of a separation between the target compound at its closest eluting by-products as possible in order to maximize product purity and load. Many che...
I often get questions from customers about the influence of flow rate on their flash chromatography. Typically, the questions are…
For many years I have been publishing a blog on flash chromatography, especially related to its theory and application. It dawned on me today that one area I have not yet addressed is the impact of th...
Normal-phase flash chromatography is an integral component of the organic synthesis workflow. Since reactions rarely generate 100% pure product, they need purification and flash chromatography is the ...
Gradient or isocratic elution, that is the question. Within flash chromatography these are the options afforded to separate reaction mixtures and natural product extracts and is the focus of this post...
Gradients, used in chromatographic methods, assist with chemical separation and elution. They begin with “weak” elution conditions and end with “strong” elution conditions.
A fairly common question I receive is "how much of my reaction mixture can I load on my flash chromatography column?". To this question there is not a cut and dry answer because of a number of variabl...
Chromatography is as much an art as it is a science. Between synthetic reaction products and natural products, the range of compounds requiring separation, purification, and isolation is broad and div...